Jute nanocrystalline cellulose enhances stress resilience in Lactobacillus rhamnosus by modulating transcriptional homeostasis.

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI:10.1016/j.ijbiomac.2025.145364

Liangbin Li, Yuanyuan Zhang, Haiyang Chi, Chenxiao Chen, Sa Yang, Xiushi Yang, Chunsheng Hou, Yanchun Deng

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Abstract

Probiotics, particularly Lactobacillus rhamnosus, are critical for gut health and immune function but encounter significant challenges from environmental stressors during production and gastrointestinal transit. This study demonstrated that jute-derived nanocrystalline cellulose (JNCC), a novel dietary fiber, at low doses (0.1-10 μg/mL), effectively protects L. rhamnosus against various stresses, including heat, oxidative, and antibiotic (such as kanamycin and ampicillin) treatment. Optimal protection was observed at 1 μg/mL JNCC, which preserved cellular morphology and viability under various stress conditions. Monosaccharide composition analysis revealed JNCC primarily comprising rhamnose (30.95 %), glucuronic acid (29.58 %), and galacturonic acid (20.61 %). Transcriptomic and phenotypic analyses further indicated that JNCC maintains probiotic resilience by significantly modulating the expression level of genes related to membrane protein transport and DNA integration pathways, thereby stabilizing transcriptional homeostasis. The work revealed JNCC as a promising low-dose prebiotic additive to enhance probiotic survival in implications for functional food and biomedical applications.

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黄麻纳米晶纤维素通过调节转录稳态增强鼠李糖乳杆菌的应激恢复能力。

益生菌，特别是鼠李糖乳杆菌，对肠道健康和免疫功能至关重要，但在生产和胃肠道运输过程中受到环境压力的重大挑战。研究表明，低剂量（0.1-10 μg/mL）的黄麻源性纳米晶纤维素（JNCC）可有效保护鼠李糖（L. rhamnosus）免受高温、氧化和抗生素（如卡那霉素和氨苄西林）等多种胁迫。1 μg/mL的JNCC对细胞的保护效果最好，在各种应激条件下均能保持细胞形态和活力。单糖组成分析显示，JNCC主要由鼠李糖（30.95 %）、葡萄糖醛酸（29.58 %）和半乳糖醛酸（20.61 %）组成。转录组学和表型分析进一步表明，JNCC通过显著调节膜蛋白转运和DNA整合途径相关基因的表达水平，从而稳定转录稳态，从而维持益生菌的恢复力。本研究揭示了JNCC作为一种有前景的低剂量益生元添加剂，在功能性食品和生物医学应用中可以提高益生菌的存活率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.