Characterisation of the genome and secretome of Phytophthoracryptogea and P.erythroseptica.

IF 5.2 1区生物学 Q1 MYCOLOGY

Ima Fungus Pub Date : 2025-06-10 eCollection Date: 2025-01-01 DOI:10.3897/imafungus.16.156195

Yuuri Hirooka, Oscar Villanueva, Ekaterina Ponomareva, Bennett L Crane, Rick D Peters, Walid Ellouze, Hai D T Nguyen

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引用次数: 0

Abstract

Phytophthoracryptogea Pethybr. & Laff. and P.erythroseptica Pethybr. are oomycetes that cause root rot diseases of multiple plant species, including serious diseases of potato. These two species of Phytophthora were originally reported in Ireland more than 100 years ago and are closely related phylogenetically and morphologically similar. Both species have wide host ranges and can hybridise with each other. In this study, we sequenced whole genomes of the ex-type strain of P.cryptogea (CBS 113.19) and the authentic strain of P.erythroseptica (P6180). The genomes of the two isolates were assembled into 52.5 Mb and 74.7 Mb, respectively. A total of 11,654 protein-encoding genes were predicted for P.cryptogea and 15,970 for P.erythroseptica. Phylogenomic analyses of 2012 single-copy orthologous genes and 99 BUSCO genes from the stramenopiles dataset confirmed that they are sister species and show that they belong to Phytophthora clade 8. The secretome analysis of P.erythroseptica P6180, P.cryptogea CBS 418.71 and P.cryptogea CBS 113.19 may provide information for future research on resistance-breeding targets and strategies for pathogen control. This genomic characterisation of the two Phytophthora species provides additional reference data that might be useful for future studies on Phytophthora genetic variation, pathogenicity and biological traits.

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Characterisation的基因组和分泌组的胸藻和红脓杆菌。

Phytophthoracryptogea Pethybr。& Laff。和红脓杆菌Pethybr。是引起多种植物根腐病的卵菌，包括马铃薯的严重病害。这两种疫霉早在100多年前就在爱尔兰被报道过，它们在系统发育和形态上有密切的关系。这两个物种都有广泛的寄主范围，并且可以相互杂交。本研究对隐孢假单胞菌前型菌株（CBS 113.19）和红脓假单胞菌原株（P6180）的全基因组进行了测序。两个分离株的基因组分别组装为52.5 Mb和74.7 Mb。共有11654个蛋白编码基因被预测为隐孢子虫，15970个蛋白编码基因被预测为红脓杆菌。对2012个单拷贝同源基因和99个BUSCO基因进行系统基因组分析，证实它们是姐妹种，属于疫霉菌第8枝。通过对红脓毒杆菌P6180、隐脓毒杆菌CBS 418.71和隐脓毒杆菌CBS 113.19的分泌组学分析，为今后研究耐药靶点和病原菌防治策略提供参考。这两种疫霉菌的基因组特征为今后疫霉菌遗传变异、致病性和生物学性状的研究提供了额外的参考数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ima Fungus Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

11.00

自引率

3.70%

发文量

审稿时长

20 weeks

期刊介绍： The flagship journal of the International Mycological Association. IMA Fungus is an international, peer-reviewed, open-access, full colour, fast-track journal. Papers on any aspect of mycology are considered, and published on-line with final pagination after proofs have been corrected; they are then effectively published under the International Code of Nomenclature for algae, fungi, and plants. The journal strongly supports good practice policies, and requires voucher specimens or cultures to be deposited in a public collection with an online database, DNA sequences in GenBank, alignments in TreeBASE, and validating information on new scientific names, including typifications, to be lodged in MycoBank. News, meeting reports, personalia, research news, correspondence, book news, and information on forthcoming international meetings are included in each issue

﻿Characterisation of the genome and secretome of Phytophthoracryptogea and P.erythroseptica.

Abstract

Characterisation of the genome and secretome of Phytophthoracryptogea and P.erythroseptica.