Prevalence and zoonotic potential of Enterocytozoon bieneusi and Giardia duodenalis in dairy cattle from Anhui Province, China.

IF 2.6 2区农林科学 Q1 VETERINARY SCIENCES

Frontiers in Veterinary Science Pub Date : 2025-06-05 eCollection Date: 2025-01-01 DOI:10.3389/fvets.2025.1613342

Falei Li, Shimei Cheng, Chaoyue He, Libing Meng, Aihong Wang, Meng Shao, Gaoxiao Xu, Huilin Zhang

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Abstract

Introduction: Enterocytozoon bieneusi (E. bieneusi) and Giardia duodenalis (G. duodenalis) are common intestinal pathogens in humans and farmed animals. There is limited data available on the positivity rates and genetic identity of E. bieneusi and G. duodenalis in dairy cattle from Anhui, China.

Methods: To understand the transmission of E. bieneusi and G. duodenalis in these animals, a total of 1,043 fecal samples were collected from cattle on five farms (Fuyang, Huainan, Huaibei, Bengbu, and Jieshou) in Anhui province of China between May 2023 and August 2024. The G. duodenalis in fecal samples was detected by nested PCR targeting a 511-bp fragment of the β-giardin (bg) gene, a 599-bp fragment of the glutamate dehydrogenase (gdh) gene, and a 530-bp fragment of the triosephosphate isomerase (tpi) gene. The E. bieneusi was detected by nested PCR targeting a 392-bp fragment of the internal transcribed spacer (ITS) of the rRNA gene.

Results: The PCR analysis revealed positivity rates of 29.7% (310/1043) for E. bieneusi and 2.8% (29/1043) for G. duodenalis. The cattle from the Bengbu farm had significantly higher positivity rates of E. bieneusi than Fuyang, Huainan, and Huaibei farms (χ ² = 61.6, df = 1, p < 0.0001; χ ² = 76.4, df = 1, p < 0.0001; χ ² = 20.6, df = 1, p < 0.0001). A total of 11 known genotypes of E. bieneusi have been identified: J (n = 154), BEB4 (n = 76), I (n = 63), CGC1 (n = 8), N (n = 2), BEB8 (n = 2), ALP1 (n = 1), BLC13 (n = 1), CHC13 (n = 1), CHN6 (n = 1), and D (n = 1). Additionally, two genotypes of G. duodenalis have been identified, including assemblage A (n = 6) and assemblage E (n = 23).

Discussion: The results indicate that known zoonotic E. bieneusi and G. duodenalis are prevalent in dairy cattle, thereby enhancing our understanding of the genetic diversity and transmission of these pathogens in these animals.

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安徽省奶牛双胞虫和十二指肠贾第鞭毛虫的流行情况及人畜共患潜力。

简介：连氏肠胞虫（E. bieneusi）和十二指肠贾第鞭毛虫（G. duodenalis）是人类和养殖动物中常见的肠道病原体。关于中国安徽奶牛中布氏乳杆菌和十二指肠乳杆菌的阳性率和遗传特性的数据有限。方法：2023年5月至2024年8月，在安徽省阜阳、淮南、淮北、蚌埠和揭寿5个养殖场采集1043份牛粪样本，以了解布氏埃希菌和十二指肠埃希菌在这些动物中的传播情况。采用巢式PCR方法，对粪便中十二指肠十二指肠螺杆菌β-栀子苷（bg）基因511-bp片段、谷氨酸脱氢酶（gdh）基因599-bp片段和三磷酸异构酶（tpi）基因530-bp片段进行检测。采用巢式PCR方法，以rRNA基因内部转录间隔段（ITS）的392 bp片段为靶点，检测了白氏伊蚊。结果：PCR检测结果显示，bienesi阳性检出率为29.7%(310/1043)，十二指肠梭菌阳性检出率为2.8%（29/1043）。牛的农场蚌埠市积极性的大肠bieneusi率要明显高于阜阳、淮南、淮北农场(χ2 = 61.6,df = 1,p χ2 = 76.4,df = 1,p χ2 = 20.6,df = 1,p E。bieneusi已确定:J (n = 154),BEB4 (n = 76),我(n = 63),CGC1 (n = 8),n (n = 2),BEB8 (n = 2),ALP1 (n = 1),BLC13 (n = 1),CHC13 (n = 1),CHN6 (n = 1),和D (n = 1)。此外，还鉴定出了两个基因型，包括组合A （n = 6）和组合E （n = 23）。讨论：结果表明，已知的人畜共患双氏肠杆菌和十二指肠肠杆菌在奶牛中普遍存在，从而增强了我们对这些病原体在这些动物中的遗传多样性和传播的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Veterinary Science Veterinary-General Veterinary

CiteScore

4.80

自引率

9.40%

发文量

1870

审稿时长

14 weeks

期刊介绍： Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy. Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field. Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.