Comprehensive UHPLC-MS/MS and GC-MS Metabolite Profiling and Anti-Inflammatory Effect of the Ethanol Extract and Essential Oil of Cymbopogon citratus (DC.) Staph Grown in Aceh, Indonesia.

IF 2.3 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Chemistry & Biodiversity Pub Date : 2025-06-20 DOI:10.1002/cbdv.202500668

Irma Sari, Mustanir Yahya, Ibrahim Jantan, Binawati Ginting, Elfahmi, Marissa Angelina, Hilda Maysarah

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Abstract

In this study, a comprehensive chemical profiling of Cymbopogon citratus metabolites grown in Aceh, Indonesia, was performed by UHPLC-ESI-QTOF-MS/MS on its 80% ethanol extract, and GC-MS on its hydrodistilled essential oil. The samples were also investigated for their anti-inflammatory activity through bovine serum albumin (BSA) protein denaturation inhibition and human red blood cell (HRBC) membrane stabilization techniques. LC-MS/MS analysis of the extract putatively identified 54 compounds, represented by 23 phenolic compounds (flavonoids, flavonoid glycosides, lignans, anthraquinone glycosides, coumarins, and a phenolic acid), 7 carboxylic acids and esters, 7 carbohydrates, 5 amino acids, 5 lipids, 2 steroids, 2 lactones, 2 alkaloids, and 1 monoterpenoid. GC-MS analysis of the essential oil indicated it was mainly made up of monoterpenoids with neral (25.89%) and geranial (49.97%) as the major compounds. The chemical contents identified in the extract and essential oil of the plant grown in Aceh were qualitatively and quantitatively different from samples reported elsewhere. Both extract and essential oil exhibited anti-inflammatory activity by stabilizing the HRBC membrane against hypotonicity-induced hemolysis and inhibited the BSA protein denaturation in a concentration-dependent manner. The anti-inflammatory activity of C. citratus was attributed mainly to the presence of bioactive compounds especially phenolic compounds and monoterpenoids.

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枸杞醇提物和精油的UHPLC-MS/MS和GC-MS综合代谢物分析及抗炎作用葡萄球菌生长在印度尼西亚的亚齐。

本研究采用UHPLC-ESI-QTOF-MS/MS对生长在印度尼西亚亚齐的Cymbopogon citratus代谢产物进行了全面的化学分析，并对其80%乙醇提取物进行了hplc - esi - qtof -MS/MS，对其水蒸馏精油进行了GC-MS分析。通过牛血清白蛋白（BSA）变性抑制和人红细胞（HRBC）膜稳定技术研究了样品的抗炎活性。通过LC-MS/MS分析，鉴定出54种化合物，包括23种酚类化合物（类黄酮、类黄酮苷、木脂素、蒽醌苷、香豆素和一种酚酸）、7种羧酸和酯类、7种碳水化合物、5种氨基酸、5种脂类、2种类固醇、2种内酯、2种生物碱和1种单萜类。GC-MS分析表明，其主要成分为单萜类化合物，主要成分为大黄酮（25.89%）和天竺葵（49.97%）。在亚齐种植的植物的提取物和精油中鉴定出的化学成分在质量和数量上与其他地方报告的样品不同。提取物和精油均表现出抗炎活性，通过稳定HRBC膜抗低压诱导的溶血，并以浓度依赖的方式抑制BSA蛋白变性。柑橘的抗炎活性主要是由于其含有生物活性成分，尤其是酚类化合物和单萜类化合物。

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来源期刊

Chemistry & Biodiversity 环境科学-化学综合

CiteScore

3.40

自引率

10.30%

发文量

475

审稿时长

2.6 months

期刊介绍： Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level. Since 2017, Chemistry & Biodiversity is published in an online-only format.