Eco-friendly Synthesis, Characterization, and Biological Evaluation of Silver Nanoparticles from Verbascum uschakense Aqueous Extract.

IF 1.2 4区 化学 Q3 CHEMISTRY, MULTIDISCIPLINARY
Mürüvvet Kurt, Funda Çet
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引用次数: 0

Abstract

Silver nanoparticles synthesized using Verbascum uschakense extract at a reaction temperature of 60 °C were characterized through multiple analytical techniques. These included ultraviolet-visible absorption spectroscopy, X-ray diffraction analysis, high-resolution transmission electron microscopy, and scanning electron microscopy coupled with energy dispersive X-ray spectroscopy. Ultraviolet-visible spectral analysis revealed a surface plasmon resonance peak at 432 nanometers, indicating the successful formation of silver nanoparticles. Microscopic analyses demonstrated that the nanoparticles were predominantly spherical in shape, with particle sizes ranging between 4 and 14 nanometers. X-ray diffraction analysis confirmed that the silver nanoparticles possessed a face-centered cubic crystal structure, as evidenced by characteristic diffraction peaks at 2θ values of 38.21°, 44.46°, 64.59°, and 77.48°, corresponding to the (111), (200), (220), and (311) planes, respectively. Regarding the biological activities, the antioxidant capacity of the aqueous extract of V. uschakense was evaluated by its ability to scavenge free radicals. At a concentration of 1 milligram per milliliter, the extract demonstrated a 86.93 percent scavenging effect against the stable radical 2,2-diphenyl-1-picrylhydrazyl. In comparison, the silver nanoparticles synthesized from the plant extract exhibited a 60.04 percent scavenging effect at the same concentration. Additionally, the silver nanoparticles showed strong inhibition in the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay, with values comparable to those obtained using the standard antioxidant TroloxThe antimicrobial properties of the biosynthesized silver nanoparticles were examined using the disc diffusion method. The nanoparticles exhibited inhibitory activity against several pathogenic microorganisms, including Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, Listeria monocytogenes, and fungal species belonging to the Candida genus. These results highlight the potential of V. uschakense-mediated silver nanoparticles as multifunctional bioactive agents with both antioxidant and antimicrobial properties. The study demonstrates that green synthesis offers a sustainable and effective approach to producing nanomaterials with potential applications in biomedical and pharmaceutical fields.

水草水提物纳米银的生态合成、表征及生物学评价。
以水芹提取物为原料,在反应温度为60 °C的条件下合成了纳米银。其中包括紫外-可见吸收光谱、x射线衍射分析、高分辨率透射电子显微镜和扫描电子显微镜结合能量色散x射线光谱。紫外-可见光谱分析显示,表面等离子体共振峰位于432纳米处,表明银纳米颗粒的成功形成。显微分析表明,纳米颗粒主要呈球形,粒径在4 ~ 14纳米之间。x射线衍射分析证实了纳米银具有面心立方晶体结构,在2θ值处的特征衍射峰分别为38.21°、44.46°、64.59°和77.48°,分别对应于(111)、(200)、(220)和(311)面。在生物活性方面,通过对自由基的清除能力来评价水仙水提物的抗氧化能力。在浓度为1毫克/毫升时,提取物对稳定自由基2,2-二苯基-1-苦味酰肼的清除效果为86.93%。相比之下,在相同浓度下,由植物提取物合成的银纳米颗粒的清除效果为60.04%。此外,银纳米粒子在2,2'-氮基-双(3-乙基苯并噻唑-6-磺酸)自由基清除实验中表现出很强的抑制作用,其值与标准抗氧化剂trolox相当。采用盘扩散法检测生物合成银纳米粒子的抗菌性能。纳米颗粒对几种病原微生物具有抑制活性,包括金黄色葡萄球菌、变形链球菌、大肠杆菌、铜绿假单胞菌、单核细胞增生李斯特菌和假丝酵母属真菌。这些结果突出了V. uschakense介导的纳米银作为具有抗氧化和抗菌特性的多功能生物活性剂的潜力。研究表明,绿色合成为制备具有潜在应用前景的纳米材料提供了一条可持续、有效的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta Chimica Slovenica
Acta Chimica Slovenica 化学-化学综合
CiteScore
2.50
自引率
25.00%
发文量
80
审稿时长
1.0 months
期刊介绍: Is an international, peer-reviewed and Open Access journal. It provides a forum for the publication of original scientific research in all fields of chemistry and closely related areas. Reviews, feature, scientific and technical articles, and short communications are welcome.
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