Black carbon modulates BDE-47-induced developmental toxicity in zebrafish larvae: Dual roles as pollutant carrier and toxic alleviator

IF 4.1 2区环境科学与生态学 Q1 MARINE & FRESHWATER BIOLOGY

Aquatic Toxicology Pub Date : 2025-06-16 DOI:10.1016/j.aquatox.2025.107460

Pan Yi , Ruixin Guo , Jinxing Wang , Yaqian Gao , Zishan Liu , Zhenhua Zhang , Jianqiu Chen , Yanhua Liu

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引用次数: 0

Abstract

Black carbon (BC), as a particulate pollutant prevalent in aquatic environments, exhibits strong adsorption affinity for polybrominated diphenyl ethers (PBDEs). Despite extensive studies on PBDEs’ neurotoxicity, the role of BC in modulating these effects remains poorly understood. Exposure to BDE-47 (2, 20, 200 μg/L) induced concentration-dependent oxidative stress, neuroglial damage, locomotor/photoaxis behavioral abnormalities, and growth retardation. Co-exposure to BC (0.5 mg/L) mitigated these effects, reducing developmental retardation, heartbeat elevation, and malformations such as pericardial oedema and spinal curvature. BC also alleviated behavioral impairment and normalized the levels of thyroid hormones (triiodothyronine and thyroxine), neurodevelopment-related proteins (growth-associated protein 43, glial fibrillary acidic protein, and sonic hedgehog factor A), and neurotransmitters (serotonin, γ-aminobutyric acid, dopamine, and norepinephrine). BC alleviated the expression disorders of HPT axis and neural-related genes induced by BDE-47. Meanwhile, adsorption-desorption experiments revealed BC’s carrier role in sequestering BDE-47, which demonstrated that BC acts as an effective adsorbent to sequester BDE-47, thereby reducing its bioavailability and alleviating its toxicity in zebrafish. These findings provide mechanistic evidence that BC mitigates PBDE-induced neurotoxicity through dual pathways: chemical adsorption and endocrine-axis modulation. This study highlights the necessity to reassess ecological risks of co-existing particulate pollutants and POPs, with critical implications for aquatic ecosystem protection and human health risk prediction.

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黑碳调节bde -47对斑马鱼幼体的发育毒性：作为污染物载体和毒性缓解剂的双重作用

黑碳（BC）是水生环境中普遍存在的颗粒污染物，对多溴联苯醚（PBDEs）具有很强的吸附亲和力。尽管对多溴二苯醚的神经毒性进行了广泛的研究，但BC在调节这些作用中的作用仍然知之甚少。暴露于BDE-47 （2,20,200 μg/L）会引起浓度依赖性氧化应激、神经胶质损伤、运动/光轴行为异常和生长迟缓。共同暴露于BC （0.5 mg/L）减轻了这些影响，减少了发育迟缓、心跳升高和畸形，如心包水肿和脊柱弯曲。BC还能减轻行为障碍，使甲状腺激素（三碘甲状腺原氨酸和甲状腺素）、神经发育相关蛋白（生长相关蛋白43、胶质纤维酸性蛋白和超音hedgehog因子A）和神经递质（血清素、γ-氨基丁酸、多巴胺和去甲肾上腺素）水平正常化。BC可减轻BDE-47诱导的HPT轴及神经相关基因的表达障碍。同时，通过吸附-解吸实验揭示了BC对BDE-47的吸附作用，说明BC是一种有效的吸附BDE-47的吸附剂，可以降低BDE-47在斑马鱼体内的生物利用度，减轻其毒性。这些发现提供了机制证据，表明BC通过化学吸附和内分泌轴调节双重途径减轻多溴二苯醚诱导的神经毒性。这项研究强调了重新评估共存的颗粒污染物和持久性有机污染物的生态风险的必要性，对水生生态系统保护和人类健康风险预测具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquatic Toxicology 环境科学-毒理学

CiteScore

7.10

自引率

4.40%

发文量

250

审稿时长

56 days

期刊介绍： Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems. Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.