Evaluation of thermoresponsive biopolymers-based composite bioinks for cartilage engineering

IF 7.6 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Materials & Design Pub Date : 2025-06-18 DOI:10.1016/j.matdes.2025.114261

Yawei Gu , Gangyu Zhang , Sébastien Pigeot , Yi Qian , Tobias Butelmann , Ivan Martin , Andrea Barbero , V. Prasad Shastri

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引用次数: 0

Abstract

Due to the low regenerative capacity of damaged cartilage, strategies to promote cartilage repair remain an evolving but unmet clinical need. 3D bioprinting (3DBP) offers a way to refine the principles of tissue engineering and generate bespoke cell-laden constructs better aligned with the biological aspects of the cartilage trauma and the anatomical site. Therefore, bioinks that possess attributes for extrusion-printing and support the cartilage matrix deposition are essential. In this study, bioinks comprising a double network of thermogelling biopolymers, namely carboxylated agarose and gelatin, were developed and characterized extensively for their rheological properties and printability. Formulations that combine printability and printed object stability were identified using an optimization strategy, and their ability to support extraceluular matrix (ECM) production in human nasal chondrocytes (hNCs) was investigated. In general, printed constructs with lower solid content favored ECM deposition, and the incorporation of gelatin improved hNCs distribution, homogeneity of type-II collagen expression, and biosynthesis of glycosaminoglycans. These findings offer valuable insights into the development of bioinks that can be translated into practical applications for cartilage engineering.

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热响应性生物聚合物基复合生物墨水在软骨工程中的应用

由于受损软骨的再生能力较低，促进软骨修复的策略仍然是一个不断发展但尚未满足的临床需求。生物3D打印（3DBP）提供了一种改进组织工程原理的方法，可以生成定制的细胞负载结构，更好地符合软骨损伤的生物学方面和解剖部位。因此，具有挤压打印特性和支持软骨基质沉积的生物墨水是必不可少的。在这项研究中，生物墨水包括热凝胶生物聚合物的双重网络，即羧化琼脂糖和明胶，被开发和广泛表征其流变性和可印刷性。使用优化策略确定了结合可打印性和打印对象稳定性的配方，并研究了它们支持人鼻软骨细胞（hNCs）中细胞外基质（ECM）生成的能力。总的来说，固体含量较低的打印结构有利于ECM沉积，明胶的加入改善了hNCs的分布、ii型胶原表达的均匀性和糖胺聚糖的生物合成。这些发现为生物墨水的发展提供了有价值的见解，可以转化为软骨工程的实际应用。

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来源期刊

Materials & Design Engineering-Mechanical Engineering

CiteScore

14.30

自引率

7.10%

发文量

1028

审稿时长

85 days

期刊介绍： Materials and Design is a multi-disciplinary journal that publishes original research reports, review articles, and express communications. The journal focuses on studying the structure and properties of inorganic and organic materials, advancements in synthesis, processing, characterization, and testing, the design of materials and engineering systems, and their applications in technology. It aims to bring together various aspects of materials science, engineering, physics, and chemistry. The journal explores themes ranging from materials to design and aims to reveal the connections between natural and artificial materials, as well as experiment and modeling. Manuscripts submitted to Materials and Design should contain elements of discovery and surprise, as they often contribute new insights into the architecture and function of matter.