Deposition of alginate-oregano nanofibres on cotton gauze for potential antimicrobial applications

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-06-18 DOI:10.1016/j.ijbiomac.2025.145372

Abimbola Oluwatayo Orisawayi , Hao Lu , Ishrat Jahan Badruddin , Prabhuraj D. Venkatraman , Nicole S. Britten , Jonathan A. Butler , Krzysztof Koziol , Sameer S. Rahatekar

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Abstract

In this study, we developed an innovative natural antibacterial medical bandage composed of electrospun nanofibres derived from alginate (SAg) and oregano essential oil (OEO). The nanofibre deposition process was systematically optimised, achieving a controlled evolution of fibre formation at intervals of 1, 2, 3, 4, and 8 h. Over time, fibre morphology has changed from a dispersed network to a densely packed, homogeneous, fibrous, fully embedding cotton gauze nanofibre. Scanning Electron Microscopy (SEM) revealed nanofibres with diameters ranging from 100 to 300 nm, 46 % measuring 100–200 nm, 37 % at 200–300 nm, and 14 % between 300 and 400 nm. Thermogravimetric Analysis (TGA) confirmed improved thermal stability in cross-linked samples. At the same time, Fourier Transform Infrared Spectroscopy (FTIR) shows the incorporation of OEO into the nanofibres shows OEO carvacrol, and thymol. Antibacterial efficacy tested inhibition zone assays against Methicillin-resistant Staphylococcus aureus (MRSA) and Listeria monocytogenes on double-layered bandages is 15 mm and 10 mm, respectively. Statistical analysis results from ANOVA confirmed that multi-layered bandages (TL-BSS) had significantly enhanced antibacterial activity compared to single-layered (SSS) and both-sided spun (BSS) configurations. Unlike conventional wound dressings, this study introduces a bioactive, nanofibre-integrated gauze with sustained antibacterial efficacy.

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海藻酸盐-牛至纳米纤维在棉纱上的沉积及其潜在的抗菌应用

在这项研究中，我们开发了一种创新的天然抗菌医用绷带，该绷带由海藻酸盐（SAg）和牛至油（OEO）衍生的静电纺纳米纤维组成。研究人员对纳米纤维沉积过程进行了系统优化，在1、2、3、4和8小时的间隔时间内实现了纤维形成的可控演变。随着时间的推移，纤维形态从分散的网络变为密集排列、均匀、纤维状、完全嵌入的棉纱纳米纤维。扫描电子显微镜（SEM）显示，纳米纤维的直径范围为100至300纳米，其中46%的直径范围为100至200纳米，37%的直径范围为200至300纳米，14%的直径范围为300至400纳米。热重分析（TGA）证实了交联样品的热稳定性得到改善。同时，傅里叶变换红外光谱（FTIR）显示，在纳米纤维中掺入OEO后，OEO中含有香芹酚和百里香酚。双层绷带对耐甲氧西林金黄色葡萄球菌（MRSA）和单核增生李斯特菌的抑菌区分别为15 mm和10 mm。方差分析结果证实，多层绷带（TL-BSS）的抗菌活性明显高于单层绷带（SSS）和双面绷带（BSS）。与传统的伤口敷料不同，本研究引入了一种具有持续抗菌功效的生物活性纳米纤维集成纱布。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.