Protective effects of pollenin B in asthma: PPAR-γ-mediated regulation of inflammatory pathways and arachidonic acid metabolism

IF 6.7 1区医学 Q1 CHEMISTRY, MEDICINAL

Phytomedicine Pub Date : 2025-06-11 DOI:10.1016/j.phymed.2025.156975

Yuanyuan Wu , Mengnan Zeng , Xinmian Jiao , Xinyi Ma , Haoyu Wang , Yanling Chen , Xiaoke Zheng , Weisheng Feng

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引用次数: 0

Abstract

Background

Asthma poses a significant global health challenge, characterized by increasing prevalence and treatment complexities. Although Ephedrae Herba has long been used in asthma therapy, its pharmacological basis and mechanism of action remain unclear.

Purpose

This study aimed to clarify the protective effects of pollenin B (PolB), a compound extracted from Ephedrae Herba, in asthmatic mice and to thoroughly explore its possible action pathways and molecular mechanisms. This study supplements pharmacological research on the anti-asthmatic effects of Ephedrae Herba and provides more information for the development of novel and highly effective drugs for the treatment of asthma.

Methods

An ovalbumin (OVA)-induced asthma model was used to evaluate the effects of PolB on asthma symptoms and lung histopathology. Furthermore, cytokine profiles in the serum and bronchoalveolar lavage fluid (BALF), immune cell populations, and serum metabolomics data were analyzed to identify the pathways involved in PolB activity. PPAR-γ was identified as a key target, and its interaction with PolB was confirmed by surface plasmon resonance (SPR) analysis. A PPAR-γ inhibitor (T0070907) was administered in vivo, and combined PPAR-γ agonist (rosiglitazone)/inhibitor (T0070907) assays were conducted in vitro to validate the mechanisms involved.

Results

PolB improved asthma symptoms, modulated cytokine levels in the serum and BALF, modulated immune cells, and influenced arachidonic acid metabolism. Further mechanistic exploration indicated that PolB mitigated airway inflammation by regulating arachidonic acid metabolism and the JAK-STAT pathway via PPAR-γ.

Conclusion

PolB exerts anti-asthmatic effects via PPAR-γ-mediated regulation of key pathways. These findings not only enrich modern pharmacological research on the anti-asthmatic properties of Ephedrae Herba but also deepen our understanding of asthma pathogenesis, thus providing new insights into the optimization of asthma prevention and treatment.

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花粉素B对哮喘的保护作用：PPAR-γ介导的炎症通路和花生四烯酸代谢的调节

哮喘是一项重大的全球健康挑战，其特点是患病率和治疗复杂性不断增加。虽然麻黄长期用于哮喘治疗，但其药理基础和作用机制尚不清楚。目的研究麻黄提取物花粉素B （PolB）对哮喘小鼠的保护作用，探讨其可能的作用途径和分子机制。本研究是对麻黄抗哮喘药理研究的补充，为开发新型高效的哮喘治疗药物提供了更多信息。方法采用卵清蛋白（OVA）诱导哮喘模型，观察PolB对哮喘症状及肺组织病理学的影响。此外，研究人员分析了血清和支气管肺泡灌洗液（BALF）、免疫细胞群和血清代谢组学数据中的细胞因子谱，以确定参与PolB活性的途径。PPAR-γ被确定为关键靶点，并通过表面等离子体共振（SPR）分析证实其与PolB的相互作用。在体内给药PPAR-γ抑制剂（T0070907），并在体外进行PPAR-γ激动剂（罗格列酮）/抑制剂（T0070907）联合试验以验证其作用机制。结果spolb可改善哮喘症状，调节血清细胞因子和BALF水平，调节免疫细胞，影响花生四烯酸代谢。进一步的机制探索表明，PolB通过PPAR-γ调节花生四烯酸代谢和JAK-STAT通路减轻气道炎症。结论polb通过PPAR-γ介导的关键通路发挥抗哮喘作用。这些发现不仅丰富了麻黄抗哮喘特性的现代药理学研究，也加深了我们对哮喘发病机制的认识，从而为优化哮喘防治提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytomedicine 医学-药学

CiteScore

10.30

自引率

5.10%

发文量

670

审稿时长

91 days

期刊介绍： Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.