Illuminating Rhodopsin Activation in Real Time.

IF 3.2 3区生物学 Q2 BIOPHYSICS

Biophysical journal Pub Date : 2025-06-18 DOI:10.1016/j.bpj.2025.06.018

Kota Katayama

引用次数: 0

Abstract

Recent advances in structural biology have enabled not only the determination of static three-dimensional structures of proteins but also the visualization of their conformational dynamics in action, offering time-resolved insights into functional transitions. Techniques such as time-resolved X-ray crystallography using X-ray free-electron lasers (XFELs), and single-particle cryo-electron microscopy (cryo-EM) combined with computational classification of structural heterogeneity, have revealed intermediate states of G protein-coupled receptors (GPCRs) and other membrane proteins at near-atomic resolution.

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实时照明视紫红质激活。

结构生物学的最新进展不仅可以确定蛋白质的静态三维结构，还可以可视化其作用中的构象动力学，为功能转变提供时间解决的见解。使用x射线自由电子激光器（XFELs）的时间分辨x射线晶体学和结合结构非均质性计算分类的单粒子冷冻电子显微镜（cro - em）等技术，已经在接近原子分辨率下揭示了G蛋白偶联受体（gpcr）和其他膜蛋白的中间状态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biophysical journal 生物-生物物理

CiteScore

6.10

自引率

5.90%

发文量

3090

审稿时长

2 months

期刊介绍： BJ publishes original articles, letters, and perspectives on important problems in modern biophysics. The papers should be written so as to be of interest to a broad community of biophysicists. BJ welcomes experimental studies that employ quantitative physical approaches for the study of biological systems, including or spanning scales from molecule to whole organism. Experimental studies of a purely descriptive or phenomenological nature, with no theoretical or mechanistic underpinning, are not appropriate for publication in BJ. Theoretical studies should offer new insights into the understanding ofexperimental results or suggest new experimentally testable hypotheses. Articles reporting significant methodological or technological advances, which have potential to open new areas of biophysical investigation, are also suitable for publication in BJ. Papers describing improvements in accuracy or speed of existing methods or extra detail within methods described previously are not suitable for BJ.