Methyltransferase gene-mediated antiproliferative effect of 1,25(OH)2D3 on neuroblastoma cells.

Revista da Associacao Medica Brasileira (1992) Pub Date : 2025-06-16 eCollection Date: 2025-01-01 DOI:10.1590/1806-9282.20241269

Okan Sancer

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Abstract

Objective: 1,25-Dihydroxyvitamin D3 is the body's active form of vitamin D and is also known as calcitriol. It has an effect on the body's immune system and is involved in the regulation of cell growth, differentiation, and apoptosis.

Methods: The effect of 1,25-Dihydroxyvitamin D3 on the viability of human neuroblastoma human neuroblastoma cell line cells was investigated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay, the effect on migration was investigated by wound healing assay, and methyltransferase genes (DNA methyltransferase 3 alpha and DNA methyltransferase 3 beta) and phosphatase and tensin homolog gene expression levels were investigated by real time polymerase chain reaction method.

Results: As a result of the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide assay, 1,25-Dihydroxyvitamin D3 IC50:265.6 nM was found. It was observed that 100 and 265.6 nM applied to human neuroblastoma cell line cells had an antiproliferative effect. In addition, DNA methyltransferase 3 alpha and DNA methyltransferase 3 beta genes, which are effective as methyltransferases, caused a change in expression levels (p<0.05), while phosphatase and tensin homolog expression levels did not change (p>0.05).

Conclusion: 1,25-Dihydroxyvitamin D3 showed antiproliferative, antimigratory activity on neuroblastoma human neuroblastoma cell line cells and decreased the expression levels of genes involved in methylation, which may be evidence of its anticancer effect.

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甲基转移酶基因介导的1,25(OH)2D3对神经母细胞瘤细胞的抗增殖作用。

目的：1,25-二羟基维生素D3是人体维生素D的活性形式，也被称为骨化三醇。它对人体的免疫系统有影响，并参与细胞生长、分化和凋亡的调节。方法：采用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑试验研究1,25-二羟维生素D3对人神经母细胞瘤细胞系细胞活力的影响，采用创面愈合试验研究其对迁移的影响，采用实时聚合酶链反应法研究甲基转移酶基因（DNA甲基转移酶3 α和DNA甲基转移酶3 β）、磷酸酶和紧张素同源基因的表达水平。结果：通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑测定，发现1,25-二羟基维生素D3 IC50:265.6 nM。观察到100和265.6 nM对人神经母细胞瘤细胞系细胞有抑制增殖作用。此外，作为甲基转移酶的DNA甲基转移酶3 α和DNA甲基转移酶3 β基因也引起了表达水平的变化（p0.05）。结论：1,25-二羟基维生素D3对人神经母细胞瘤细胞系细胞具有抗增殖、抗迁移的作用，可降低甲基化相关基因的表达水平，这可能是其抗癌作用的证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Revista da Associacao Medica Brasileira (1992)

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