Stromal Vascular Fraction-Derived Vasculogenesis Is Associated with the Formation of Lymphatic Endothelial Cell Structures.

Stem cells and development Pub Date : 2025-07-01 Epub Date: 2025-06-19 DOI:10.1089/scd.2024.0210
Nien-Wen Hu, Hulan Shang, Sam Kogan, Ramon Llull, Dmitry O Traktuev, Adam J Katz, Walter L Murfee
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Abstract

Therapies aimed at manipulating microvasculature require the ability to generate both blood and lymphatic vessels. Adipose-derived stromal vascular fraction (SVF), consisting of endothelial cells, progenitor cells, pericytes, smooth muscle cells, fibroblasts, and immune cells, has emerged as a heterogeneous cell composition able to promote blood vessel formation and growth, but whether SVF forms lymphatic vessels remains unknown. The objective of this study was to evaluate whether SVF can form lymphatic vessels. SVF was isolated from C57BL/6 mouse inguinal adipose tissue, characterized for prevalence of blood (PECAM+) and lymphatic (Prox1+, Podoplanin+, LYVE-1+) endothelial cells and cultured with avascular mouse mesentery tissues for up to 9 days. The presence of lymphatic endothelial cells in SVF is supported by the percentages of PECAM+ cells that are also positive for lymphatic markers. By day 1 after SVF seeding, cells established PECAM+ segments, and by day 3 cell clusters with segment extensions were observed. At later time points, segments established network of blood vessels. In parallel, a subset of structures positive for lymphatic marker labeling and characterized by a rounded shape (termed "blebs") connected with nearby SVF-derived blood vessel and were changing shape over time. Our findings provoke a new research area focused on the ability for SVF to form lymphatic vessels.

基质血管部分衍生的血管发生与淋巴内皮细胞结构的形成有关。
旨在控制微血管系统的治疗需要同时产生血液和淋巴管的能力。脂肪源性基质血管组分(SVF)由内皮细胞、祖细胞、周细胞、平滑肌细胞、成纤维细胞和免疫细胞组成,已成为一种异质细胞组成,能够促进血管的形成和生长,但SVF是否形成淋巴管尚不清楚。本研究的目的是评估SVF是否能形成淋巴管。从C57BL/6小鼠腹股沟脂肪组织中分离出SVF,其特征是血液(PECAM+)和淋巴(Prox1+, Podoplanin+, LYVE-1+)内皮细胞普遍存在,并与无血管小鼠肠系膜组织培养达9天。淋巴内皮细胞在SVF中的存在得到PECAM阳性细胞百分比的支持,PECAM阳性细胞在淋巴标志物中也呈阳性。SVF接种后第1天,细胞形成PECAM+片段,第3天观察到细胞群片段延伸。在较晚的时间点,各节段建立血管网络。与此同时,淋巴标记物标记阳性的结构子集,其特征是圆形(称为“水泡”)与附近的svf衍生血管相连,并随着时间的推移而改变形状。我们的发现引发了一个新的研究领域,即SVF形成淋巴管的能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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