The impact of Caralluma munbyana extracts on Streptococcus mutans biofilm formation.

IF 1.5 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Frontiers in dental medicine Pub Date : 2025-06-04 eCollection Date: 2025-01-01 DOI:10.3389/fdmed.2025.1575161
Turki Alshehri, Israa Alkhalifah, Areeb Alotaibi, Alaa F Alsulaiman, Abdullah Al Madani, Basil Almutairi, Abdulrahman A Balhaddad
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引用次数: 0

Abstract

Background/objectives: Caralluma plants have a wide range of anti-inflammatory and antimicrobial activities. This study aims to assess the antibacterial effect of water, methanol, and ethanol extracts of Caralluma munbyana against Streptococcus mutans biofilms.

Methods: Three extracts of C. munbyana were prepared using water, methanol, and ethanol. Multiple concentrations ranging between 2.93 and 93.75 mg/ml were achieved, alongside a control group with no extract, and incubated with an overnight culture of S. mutans. In the following day, the total absorbance was measured at 595 nm. Then, the biofilms were fixed and stained with 0.5% crystal violet to measure the biofilm absorbance at 490 nm. One-way ANOVA and Tukey's post-hoc tests were applied to identify which specific concentrations differed from the control.

Results: C. munbyana methanol and ethanol extracts significantly affected the total absorbance of S. mutans (P ≤ 0.001) at 46.87 and 93.75 mg/ml. For biofilm inhibition, C. munbyana water extract was effective (P ≤ 0.001) in reducing the biofilm growth at 23.44 (1.34 ± 0.08), 46.87 (1.31 ± 0.15), and 93.75 (1.04 ± 0.07) mg/ml when compared to the control (1.58 ± 0.11). More reduction was observed among methanol and ethanol extracts, as C. munbyana methanol extract significantly (P ≤ 0.001) inhibited the S. mutans biofilm growth at 23.44 (0.99 ± 0.15), 46.87 (0.12 ± 0.02), and 93.75 (0.09 ± 0.01) mg/ml. Similarly, C. munbyana ethanol extract's biofilm inhibition was observed at the concentrations of 23.44 (0.45 ± 0.12), 46.87 (0.10 ± 0.02), and 93.75 (0.09 ± 0.04) mg/ml.

Conclusion: These findings suggest that C. munbyana possesses antibacterial properties against S. mutans biofilms, particularly through its methanol and ethanol extracts.

绿豆提取物对变形链球菌生物膜形成的影响。
背景/目的:芥花属植物具有广泛的抗炎和抗菌活性。摘要本研究旨在探讨绿豆水、甲醇和乙醇提取物对变形链球菌生物膜的抑菌作用。方法:用水、甲醇和乙醇制备三种蒙白草提取物。在2.93和93.75 mg/ml之间的多个浓度范围内,与不含提取物的对照组一起,与变形链球菌过夜培养。第二天在595 nm处测定总吸光度。然后将生物膜固定,用0.5%结晶紫染色,测定生物膜在490 nm处的吸光度。采用单因素方差分析和Tukey事后检验来确定哪些特定浓度与对照不同。结果:木参甲醇和乙醇提取物对变形链球菌总吸光度影响显著(P≤0.001),分别为46.87和93.75 mg/ml;在抑制生物膜生长方面,与对照组(1.58±0.11)相比,孟山棠水提物对生物膜生长的抑制作用分别为23.44(1.34±0.08)、46.87(1.31±0.15)和93.75(1.04±0.07)mg/ml (P≤0.001)。甲醇和乙醇提取物对变形链球菌生物膜生长的抑制作用显著(P≤0.001),分别为23.44(0.99±0.15)、46.87(0.12±0.02)和93.75(0.09±0.01)mg/ml。同样,在23.44(0.45±0.12)、46.87(0.10±0.02)和93.75(0.09±0.04)mg/ml浓度下,青花草乙醇提取物对生物膜有抑制作用。结论:munbyana对S. mutans生物膜具有一定的抑菌作用,特别是其甲醇和乙醇提取物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
2.10
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