Effect of Air Flow Rate Over Time on SARS-CoV-2 Viability During Representative Sampling Using MD8 Airport Air Sampler.

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection Pub Date : 2025-06-16 DOI:10.1016/j.jhin.2025.06.002

Ian Nicholls, Okechukwu Onianwa, Sean Collins, Antony Spencer, Simon Parks, Barry Atkinson, Thomas Pottage

引用次数: 0

Abstract

Studies involving air sampling for SARS-CoV-2 commonly return either negative data or non-viable virus. We assessed the desiccative impact of time parameters during air sampling by aerosolising SARS-CoV-2 onto gelatine Sartorius MD8 AirPort filters; contaminated filters were then attached to a Sartorius MD8 AirPort and run at 50L/min up to 40 minutes. These filters were then analysed by viral plaque assay and qRT-PCR, results showed no significant difference in recovery of infectious virus or genomic copy numbers with increasing air sampled. These results show that sampling for 40 minutes or less with a Sartorius MD8 AirPort does not reduce the viability of SARS-CoV-2.

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MD8机场空气采样器代表性采样过程中空气流量随时间变化对SARS-CoV-2活力的影响

涉及SARS-CoV-2空气采样的研究通常返回阴性数据或无活病毒。我们通过将SARS-CoV-2雾化到明胶Sartorius MD8机场过滤器上，评估了空气采样过程中时间参数的干燥影响；然后将污染的过滤器连接到Sartorius MD8 AirPort上，以50L/min的速度运行40分钟。然后用病毒空斑试验和qRT-PCR分析这些过滤器，结果显示随着空气采样的增加，传染性病毒的恢复或基因组拷贝数没有显着差异。这些结果表明，使用赛多利斯MD8 AirPort取样40分钟或更短时间不会降低SARS-CoV-2的生存能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Hospital Infection 医学-传染病学

CiteScore

12.70

自引率

5.80%

发文量

271

审稿时长

19 days

期刊介绍： The Journal of Hospital Infection is the editorially independent scientific publication of the Healthcare Infection Society. The aim of the Journal is to publish high quality research and information relating to infection prevention and control that is relevant to an international audience. The Journal welcomes submissions that relate to all aspects of infection prevention and control in healthcare settings. This includes submissions that: provide new insight into the epidemiology, surveillance, or prevention and control of healthcare-associated infections and antimicrobial resistance in healthcare settings; provide new insight into cleaning, disinfection and decontamination; provide new insight into the design of healthcare premises; describe novel aspects of outbreaks of infection; throw light on techniques for effective antimicrobial stewardship; describe novel techniques (laboratory-based or point of care) for the detection of infection or antimicrobial resistance in the healthcare setting, particularly if these can be used to facilitate infection prevention and control; improve understanding of the motivations of safe healthcare behaviour, or describe techniques for achieving behavioural and cultural change; improve understanding of the use of IT systems in infection surveillance and prevention and control.