Evaluation, Characterization of Antioxidant Activity for Novel Benzothiazole Derivatives.

Abstract

The present study focuses on the design, synthesis, in-silico studies, characterization, and evaluation of the antidiabetic activity of nitrogen and sulphur-containing heterocyclic compounds, specifically benzothiazole derivatives (2a-2e). The synthesized compounds were characterized using spectroscopic techniques such as IR, NMR, and mass spectrometry. Their biological activity was assessed through molecular docking studies, which revealed strong binding interactions with target proteins, with compound 2a exhibiting the highest docking score (-8.7). Swiss ADME analysis was performed to evaluate pharmacokinetic properties, indicating good drug-likeness and bioavailability. In vitro antidiabetic activity was assessed using the α-amylase inhibition method, where compounds 2a and 2e demonstrated significant inhibitory potential with IC₅₀ values of 22.95±2.50µg/mL and 27.80±3.00µg/mL, respectively. These findings suggest that benzothiazole derivatives, particularly compound 2a, hold promise as potential antioxidant agents, warranting further investigation for therapeutic applications.