Oral Microbiota Dysbiosis Initiated by Chronic Colitis and the Possible Role in Oral Mucosa Changes.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-06-19 DOI:10.1111/odi.15344

Zhuo Wang, Minghui Wei, Jian Wan, Wenfang He, Jiaming Zhou, Yujie Zhang, Yi Liu, Yanxing Liu, Dan Liu, Qinglin Zhu, Xinwen Wang, Kaichun Wu

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引用次数: 0

Abstract

Objective: To investigate oral microbiota dysbiosis and cytopathological changes in oral mucosa of murine chronic colitis model and the correlation between them.

Methods: Dextran sodium sulfate (DSS) induced chronic colitis was established in SPF C57BL/6 male mice, oral microbiome characterization was performed using 16S rRNA gene sequencing, and cytopathological and immunohistochemistry assessment was performed in oral mucosa.

Results: When chronic colitis was induced, the overall microbial composition of the oral microbiome was altered with increased abundance in phylum Proteobacteria (82.2%), Actinobacteria (2.6%) and decreased abundance in Firmicutes (12.7%), Bacteroidetes (1.1%). Among the top 10 most abundance genera, Streptococcus was the only genera significantly decreased in colitis mice oral cavity. Meanwhile, oral epithelial hyperplasia was identified in the murine chronic colitis model, and the ki67 expression was significantly upregulated in oral epithelium (p < 0.05). The chronic course of colitis did not lead to obvious inflammatory infiltration in the oral mucosa. Spearman analysis indicated a strong inverse correlation (r = -0.52, p = 0.03) between oral Streptococcus and epithelium thickness.

Conclusions: The chronic colitis mice displayed epithelial hyperplasia in the oral mucosa without obvious inflammatory infiltration, which might be associated with oral dysbiosis, especially a decreased abundance of Streptococcus.

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慢性结肠炎引起的口腔微生物群失调及其在口腔黏膜变化中的可能作用。

目的：探讨慢性结肠炎模型小鼠口腔菌群失调与口腔黏膜细胞病理改变的关系。方法：建立Dextran sodium sulfate （DSS）诱导的SPF C57BL/6雄性小鼠慢性结肠炎模型，采用16S rRNA基因测序法进行口腔微生物组鉴定，并对口腔黏膜进行细胞病理学和免疫组织化学评价。结果：慢性结肠炎诱导后，口腔菌群总体微生物组成发生改变，变形菌门（82.2%）、放线菌门（2.6%）丰度增加，厚壁菌门（12.7%）、拟杆菌门（1.1%）丰度降低。在前10个丰度最高的属中，链球菌是唯一在结肠炎小鼠口腔中显著减少的属。同时，小鼠慢性结肠炎模型发现口腔上皮增生，ki67在口腔上皮中表达明显上调(p)。结论：慢性结肠炎小鼠口腔黏膜上皮增生，无明显炎症浸润，可能与口腔生态失调有关，尤其是链球菌丰度降低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.