Feedback Regulation of sPLA2-COX/5-LOX-Ca2+ in Seminal Plasma and Its Impact on Sperm Quality Parameters.

IF 4.2 2区医学 Q2 IMMUNOLOGY

Journal of Inflammation Research Pub Date : 2025-06-13 eCollection Date: 2025-01-01 DOI:10.2147/JIR.S523172

Yongjie Liu, Liang Dai, Fan Zhang, Yang Liu, Xu Li, Wenzhi Ma

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Abstract

Background: Male reproductive health is of growing concern. Sperm quality declines due to multiple factors. The role of AA metabolic network in sperm quality is unclear, and AA/COX's protection under heat stress needs study.

Aim: This study aimed to investigate the relationship between seminal plasma arachidonic acid (AA) metabolic network markers and sperm quality, as well as explore the protective effects of AA and cyclooxygenase (COX) on sperm under heat stress.

Methods: We analyzed 164 seminal plasma samples from 164 male infertility patients, categorized by sperm concentration and motility (PR < 32% vs PR ≥ 32%). A heat-stressed weak spermatogenesis model (37-42°C) was established, and AA/COX were added in vitro to assess their impact on sperm quality.

Results: Significant correlations were found between AA pathway markers (PL, sPLA2, AA, COX1/2, PGE1, PGF2α, 5-LOX, LTB4) and sperm parameters (motility, acrosome reaction, mitochondrial function, DNA fragmentation). A predictive model combining PL, AA, and COX1 effectively assessed sperm quality. In vitro, 100 pg AA ± 300 pg COX1 protected sperm at 42°C by upregulating COX2, PGE1, and PGF2α while reducing LOX/LTB4 and modulating Ca²⁺ levels, improving acrosome reactivity and reducing oxidative stress.

Conclusion: Seminal plasma AA metabolism strongly influences sperm quality, likely via sPLA2-COX/5-LOX-Ca²⁺ feedback mechanisms. The PL-AA-COX1 model may serve as a sperm quality predictor, and AA/COX1 supplementation could protect sperm under heat stress.

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精浆sPLA2-COX/5-LOX-Ca2+的反馈调控及其对精子质量参数的影响

背景：男性生殖健康日益受到关注。精子质量下降是由多种因素造成的。AA代谢网络在精子质量中的作用尚不清楚，AA/COX在热应激下的保护作用有待研究。目的：研究热应激条件下精浆花生四烯酸（AA）代谢网络标志物与精子质量的关系，探讨AA和环氧合酶（COX）对精子的保护作用。方法：对164例男性不育症患者的164份精浆样本进行分析，按精子浓度和活力进行分类（PR < 32% vs PR≥32%）。建立热应激弱精子发生模型（37 ~ 42℃），体外添加AA/COX，评估其对精子质量的影响。结果：AA途径标记（PL、sPLA2、AA、COX1/2、PGE1、PGF2α、5-LOX、LTB4）与精子参数（运动性、顶体反应、线粒体功能、DNA片段化）存在显著相关性。结合PL、AA和COX1的预测模型有效地评估了精子质量。在体外，在42°C条件下，100 pg AA±300 pg COX1通过上调COX2、PGE1和PGF2α，同时降低LOX/LTB4和调节Ca²+水平，提高顶体反应性和降低氧化应激来保护精子。结论：精浆AA代谢可能通过sPLA2-COX/5-LOX-Ca²+反馈机制影响精子质量。PL-AA-COX1模型可以作为精子质量的预测因子，AA/COX1补充可以保护热应激下的精子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Inflammation Research Immunology and Microbiology-Immunology

CiteScore

6.10

自引率

2.20%

发文量

658

审稿时长

16 weeks

期刊介绍： An international, peer-reviewed, open access, online journal that welcomes laboratory and clinical findings on the molecular basis, cell biology and pharmacology of inflammation.