Mitigation of antibiotic resistance: the efficiency of a hybrid subsurface flow constructed wetland in the removal of resistant bacteria in wastewater.

IF 3.4 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

International Journal of Phytoremediation Pub Date : 2025-06-19 DOI:10.1080/15226514.2025.2516250

Amina Farrukh Alavi, Sara Malik, Safia Ahmed, Bilal Ahmed, Muhammad Salman, Ayesha Farooq, Iffat Naz

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Abstract

This research investigates the effectiveness of a lab-scale hybrid subsurface flow constructed wetland (HSSFCW) for removing wastewater contaminants, including antibiotic-resistant bacteria (ARB), genes (ARGs) and antibiotics. The results suggested that HSSFCW demonstrated a high removal efficiency for COD (89%) and BOD (88.9%), while lower efficiencies were observed for salts, TDS, EC, and TKN. Further, various bacteria such as Enterobacter cloacae, Serratia liquefaciens and Serratia odorifera were detected in the plant rhizosphere, while Acinetobacter baumanii and Staphylococcus spp. were identified as biofilm formers on the wetland media. The mean removal efficiency of 70.44, 65.99, 70.66 and 51.49% was observed for total heterotrophic bacteria; Cefixime (Cef)-, Ciprofloxacin (Cip)-, and Linezolid (Lzd)-resistant bacteria. Upon chlorination of effluent samples, Cef-, Cip- and Lzd-resistant bacteria were effectively inactivated at 30, 15 and 7.5 mg Cl₂ min/L, respectively. The wetland achieved a removal efficiency of 83.85% for Cip and 100% for Lzd at week 12 with p = 0.040 and p < 0.001, respectively. Further, a log reduction of 0.66 for 16S, 0.82 for blaTEM, 0.61 for blaCTX, and 0.48 for blaOXA was observed. Thus, HSSFCW was observed to be efficient in removing organic contaminants, ARBs, ARGs and antibiotics from domestic wastewater and can be upgraded under natural environments.

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缓解抗生素耐药性：混合地下流人工湿地去除废水中耐药细菌的效率。

本研究探讨了实验室规模的混合型地下流人工湿地（HSSFCW）去除废水污染物的有效性，包括抗生素耐药菌（ARB）、基因（ARGs）和抗生素。结果表明，HSSFCW对COD（89%）和BOD（88.9%）的去除率较高，而对盐类、TDS、EC和TKN的去除率较低。此外，在植物根际中检测到阴沟肠杆菌、液化沙雷菌和气味沙雷菌等多种细菌，而在湿地培养基中鉴定出鲍曼不动杆菌和葡萄球菌等生物膜形成菌。对总异养菌的平均去除率分别为70.44、65.99、70.66和51.49%；头孢克肟(Cef)-，环丙沙星(Cip)-和利奈唑胺（Lzd）耐药细菌。对出水样品进行氯化处理后，分别在30、15和7.5 mg Cl2 min/L的浓度下，对Cef、Cip和lzd耐药菌有效灭活。第12周，该湿地对Cip和Lzd的去除率分别为83.85%和100%，p = 0.040和p = 16S，对blaTEM的去除率为0.82，对blaCTX的去除率为0.61，对blaOXA的去除率为0.48。因此，HSSFCW可以有效去除生活废水中的有机污染物、arb、arg和抗生素，并且可以在自然环境下进行升级。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Phytoremediation 环境科学-环境科学

CiteScore

7.60

自引率

5.40%

发文量

145

审稿时长

3.4 months

期刊介绍： The International Journal of Phytoremediation (IJP) is the first journal devoted to the publication of laboratory and field research describing the use of plant systems to solve environmental problems by enabling the remediation of soil, water, and air quality and by restoring ecosystem services in managed landscapes. Traditional phytoremediation has largely focused on soil and groundwater clean-up of hazardous contaminants. Phytotechnology expands this umbrella to include many of the natural resource management challenges we face in cities, on farms, and other landscapes more integrated with daily public activities. Wetlands that treat wastewater, rain gardens that treat stormwater, poplar tree plantings that contain pollutants, urban tree canopies that treat air pollution, and specialized plants that treat decommissioned mine sites are just a few examples of phytotechnologies.