Lacticaseibacillus salsurae sp. nov. and Levilactobacillus muriae sp. nov., isolated from traditional Chinese pickle.

IF 2 3区生物学 Q4 MICROBIOLOGY

International journal of systematic and evolutionary microbiology Pub Date : 2025-06-01 DOI:10.1099/ijsem.0.006816

Si-Tong Song, Ting-Yu Wang, Xin-Yu Zhu, Chun Tao Gu

{"title":"Lacticaseibacillus salsurae sp. nov. and Levilactobacillus muriae sp. nov., isolated from traditional Chinese pickle.","authors":"Si-Tong Song, Ting-Yu Wang, Xin-Yu Zhu, Chun Tao Gu","doi":"10.1099/ijsem.0.006816","DOIUrl":null,"url":null,"abstract":"Two novel bacteria (designated N501-2T and N40-8-2T) belonging to the genera Lacticaseibacillus and Levilactobacillus were isolated from traditional Chinese pickle ('Suan cai') and identified. Strain N501-2T was phylogenetically related to the type strains of Lacticaseibacillus baoqingensis, Lacticaseibacillus porcinae, Lacticaseibacillus manihotivorans and Lacticaseibacillus jixiensis, having 98.7-99.4% 16S rRNA gene sequence similarities. Strain N40-8-2T was phylogenetically related to the type strains of Levilactobacillus fuyuanensis, Levilactobacillus parabrevis, Levilactobacillus tujiorum, Levilactobacillus hammesii, Levilactobacillus senmaizukei and Levilactobacillus tangyuanensis, having 98.4-99.4% 16S rRNA gene sequence similarities. Strain N501-2T had 74.0-84.2% ANI (average nucleotide identity), 20.8-28.4% dDDH (digital DNA-DNA hybridization) and 73.5-86.8% AAI (average amino acid identity) values with L. manihotivorans DSM 13343T, L. porcinae JCM 19617T, L. jixiensis N163-3-2T and L. baoqingensis 47-3T. Analyses based on whole-genome sequences indicated that strain N40-8-2T was most closely related to the type strains of L. fuyuanensis, L. parabrevis, L. tujiorum and L. hammesii, having less than 87.4% ANI, 33.6% dDDH and 93.2% AAI values. Based upon the data obtained in the present study, two novel species, Lacticaseibacillus salsurae sp. nov. and Levilactobacillus muriae sp. nov., are proposed, and the type strains are N501-2T (=CCTCC AB 2024124T=JCM 37150T=LMG 33663T) and N40-8-2T (=CCTCC AB 2024128T=JCM 37001T=LMG 33659T), respectively.","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 6","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006816","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Two novel bacteria (designated N501-2^T and N40-8-2^T) belonging to the genera Lacticaseibacillus and Levilactobacillus were isolated from traditional Chinese pickle ('Suan cai') and identified. Strain N501-2^T was phylogenetically related to the type strains of Lacticaseibacillus baoqingensis, Lacticaseibacillus porcinae, Lacticaseibacillus manihotivorans and Lacticaseibacillus jixiensis, having 98.7-99.4% 16S rRNA gene sequence similarities. Strain N40-8-2^T was phylogenetically related to the type strains of Levilactobacillus fuyuanensis, Levilactobacillus parabrevis, Levilactobacillus tujiorum, Levilactobacillus hammesii, Levilactobacillus senmaizukei and Levilactobacillus tangyuanensis, having 98.4-99.4% 16S rRNA gene sequence similarities. Strain N501-2^T had 74.0-84.2% ANI (average nucleotide identity), 20.8-28.4% dDDH (digital DNA-DNA hybridization) and 73.5-86.8% AAI (average amino acid identity) values with L. manihotivorans DSM 13343^T, L. porcinae JCM 19617^T, L. jixiensis N163-3-2^T and L. baoqingensis 47-3^T. Analyses based on whole-genome sequences indicated that strain N40-8-2^T was most closely related to the type strains of L. fuyuanensis, L. parabrevis, L. tujiorum and L. hammesii, having less than 87.4% ANI, 33.6% dDDH and 93.2% AAI values. Based upon the data obtained in the present study, two novel species, Lacticaseibacillus salsurae sp. nov. and Levilactobacillus muriae sp. nov., are proposed, and the type strains are N501-2^T (=CCTCC AB 2024124^T=JCM 37150^T=LMG 33663^T) and N40-8-2^T (=CCTCC AB 2024128^T=JCM 37001^T=LMG 33659^T), respectively.

查看原文本刊更多论文

从传统泡菜中分离得到的咸味乳杆菌和酸味乳酸杆菌。

从酸菜中分离到两株乳酸杆菌属和乳酸杆菌属的新型细菌N501-2T和N40-8-2T，并进行了鉴定。菌株N501-2T与保清乳杆菌、猪乳杆菌、manihotivor乳杆菌和鸡西乳杆菌型菌株有亲缘关系，16S rRNA基因序列相似度为98.7 ~ 99.4%。菌株N40-8-2T与福源乳酸杆菌、副长乳酸杆菌、土家乳酸杆菌、哈梅斯乳酸杆菌、森maizukei乳酸杆菌和汤源乳酸杆菌类型菌株在系统发育上有亲缘关系，16S rRNA基因序列相似度为98.4% ~ 99.4%。菌株N501-2T与马齿虎L. DSM 13343T、猪L. JCM 19617T、鸡西L. N163-3-2T和保青L. 47-3T的ANI（平均核苷酸同源性）值为74.0 ~ 84.2%,dDDH（数字DNA-DNA杂交）值为20.8 ~ 28.4%，AAI（平均氨基酸同源性）值为73.5 ~ 86.8%。全基因组序列分析表明，菌株N40-8-2T与阜远L.、副abrevis L.、土家L.和哈默氏L.型菌株亲缘关系最密切，ANI值小于87.4%，dDDH值小于33.6%，AAI值小于93.2%。根据本研究获得的资料，提出了salsurae lactobacillus sp. nov和muriae levactobacillus sp. nov两个新种，类型菌株分别为N501-2T （=CCTCC AB 2024124T=JCM 37150T=LMG 33663T）和N40-8-2T （=CCTCC AB 2024128T=JCM 37001T=LMG 33659T）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International journal of systematic and evolutionary microbiology 生物-微生物学

CiteScore

5.20

自引率

21.40%

发文量

426

审稿时长

1 months

期刊介绍： Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.