Electroacupuncture alleviates Parkinson's disease by inhibiting the NLRP3 inflammasome pathway.

IF 1.7 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

American journal of translational research Pub Date : 2025-05-15 eCollection Date: 2025-01-01 DOI:10.62347/PGKC2376

Mengru Kang, Jianbin Cai, Weimin Yi, Bin Zhang, Liang Ai, Baile Ning, Jiayi Dong, Liudan Chen, Wenbin Fu

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Abstract

Objectives: To explore the therapeutic effects and potential mechanism of electroacupuncture (EA) in a Parkinson's disease (PD) mouse model.

Methods: C57BL/6 mice were randomly assigned to control, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), MPTP + sham EA, and MPTP + EA groups. PD was induced by MPTP. EA was applied at Baihui (GV20), Hegu (LI4), and Taichong (LR3). After 20 days of treatment, behavioral tests including the open field test, rotarod test, and Morris water maze were conducted. Dopaminergic neuron survival, apoptosis, and expression of inflammatory markers were assessed.

Results: MPTP-induced mice exhibited impaired motor and cognitive performance, increased brain apoptosis, reduced dopaminergic neurons, and elevated expression of α-synuclein, NLRP3, IL-1β, IL-18, caspase-1, and gasdermin D. These changes were not significantly altered by sham EA. In contrast, EA significantly improved motor and cognitive function, reduced apoptosis, preserved dopaminergic neurons, increased tyrosine hydroxylase expression, and suppressed NLRP3 inflammasome activation.

Conclusion: EA mitigates PD symptoms and exerts neuroprotective effects by inhibiting NLRP3 inflammasome-mediated neuroinflammation.

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电针通过抑制NLRP3炎性体通路缓解帕金森病。

目的：探讨电针（EA）对帕金森病（PD）小鼠模型的治疗作用及其可能机制。方法：将C57BL/6小鼠随机分为对照组、1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）组、MPTP +假EA组和MPTP + EA组。MPTP诱导PD。EA应用于百汇（GV20）、合谷（LI4）和太中（LR3）。治疗20 d后，进行行为学试验，包括空地试验、轮棒试验和Morris水迷宫。评估多巴胺能神经元的存活、凋亡和炎症标志物的表达。结果：mptp诱导小鼠运动和认知能力受损，脑凋亡增加，多巴胺能神经元减少，α-突触核蛋白、NLRP3、IL-1β、IL-18、caspase-1和gasdermin d的表达升高，假性EA未显著改变这些变化。相反，EA可显著改善运动和认知功能，减少细胞凋亡，保存多巴胺能神经元，增加酪氨酸羟化酶表达，抑制NLRP3炎性体活化。结论：EA通过抑制NLRP3炎性小体介导的神经炎症，减轻PD症状并发挥神经保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of translational research ONCOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL

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552

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