Gonadal Lipid Storage in Mytilus coruscus: A Comprehensive Gene Network and Key Gene Discovery.

IF 2.8 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zhenqi Xin, Hao Wang, Bingqi Wei, Pengzhi Qi, Xiaojun Yan, Zhi Liao, Baoying Guo, Weifeng Wang
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引用次数: 0

Abstract

The hard-shelled mussel (Mytilus coruscus), a commercially vital bivalve in China, accumulates lipids predominantly in its gonads, the species' primary edible tissue. Understanding the molecular mechanisms underlying gonad-specific lipid storage is critical for improving reproductive efficiency and aquaculture yield. This study employs comparative transcriptomic analysis of multiple tissues (gonad, gill, mantle, foot, hemolymph) to pinpoint key regulatory genes involved in lipid deposition. Through weighted gene co-expression network analysis (WGCNA), vitellogenin (VG), perilipin (PLIN), and transmembrane protein (TM) were identified as hub genes in gonadal lipid regulation. Genomic characterization revealed 13 VG and three PLIN family members in M. coruscus, which displayed conserved structural motifs and were syntenic with related bivalves, underscoring their functional significance. Phylogenetic analyses further highlighted the evolutionary conservation of these lipid-associated genes across marine invertebrates. Concurrently, sex-specific metabolic divergence was investigated. Physiological validation demonstrated that ovarian crude fat content exceeded testicular levels by 36%, corroborated histologically by larger, more stable lipid droplets in female gonads. Sex-specific expression profiling uncovered pronounced divergence: VG and PLIN were markedly enriched in ovaries, whereas glucose-6-phosphatase (G6P), a driver of energy catabolism, was elevated in testes. This study provides a molecular framework for understanding reproductive lipid metabolism in bivalves, offering biomarkers to refine broodstock management and aquaculture practices.

贻贝性腺脂质储存:一个全面的基因网络和关键基因的发现。
硬壳贻贝(Mytilus coruscus)是中国一种具有重要商业价值的双壳类动物,它主要在性腺中积累脂质,性腺是该物种的主要可食用组织。了解性腺特异性脂质储存的分子机制对提高生殖效率和水产产量至关重要。本研究通过对多个组织(性腺、鳃、地幔、足、血淋巴)的比较转录组学分析来确定参与脂质沉积的关键调控基因。通过加权基因共表达网络分析(WGCNA),确定卵黄原蛋白(VG)、卵黄原蛋白(PLIN)和跨膜蛋白(TM)为性腺脂质调节的枢纽基因。基因组鉴定结果显示,褐条蚌中有13个VG家族成员和3个PLIN家族成员,这些成员均表现出保守的结构基序,与相关双壳类同源,强调了它们的功能意义。系统发育分析进一步强调了这些脂类相关基因在海洋无脊椎动物中的进化保护。同时,研究了性别特异性代谢差异。生理验证表明,卵巢粗脂肪含量超过睾丸水平36%,组织学上证实,雌性性腺中更大、更稳定的脂滴。性别特异性表达谱揭示了明显的差异:VG和PLIN在卵巢中显著富集,而葡萄糖-6-磷酸酶(G6P),一种能量分解代谢的驱动因子,在睾丸中升高。本研究为了解双壳类动物的生殖脂质代谢提供了分子框架,为改进亲鱼管理和水产养殖实践提供了生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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