Assessment of a rapid immunochromatographic test as potential tool for strongyloidiasis diagnosis using samples from an endemic area in Brazil

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-06-17 DOI:10.1016/j.diagmicrobio.2025.116958

Henrique Tomaz Gonzaga , Vanessa da Silva Ribeiro , Rahmah Noordin , Nor Suhada Anuar

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引用次数: 0

Abstract

Despite the inclusion of strongyloidiasis as a neglected tropical disease and the mortality of the hyperinfection and disseminated forms of this disease, accurate diagnostic tools are still needed. This study evaluated SsRapid® as a candidate for a rapid diagnostic test for strongyloidiasis using well-characterized serum samples from an endemic area in Brazil. SsRapid®, a prototype immunochromatographic test that detects IgG4 anti-S. stercoralis, specifically using the recombinant antigen NIE SsRapid® was performed using 140 samples from individuals with confirmed strongyloidiasis, other parasitic diseases, or healthy/asymptomatic. Diagnostic parameters of sensitivity (Se), specificity (Sp), and positive and negative likelihood ratios (LR+ and LR-) were calculated. The sensitivity in samples from strongyloidiasis patients was 90 %, among the samples from patients with other parasitic diseases positivity was 17.5 % and 15 % in healthy individuals. The positive and negative likelihood ratios were 5.56 and 0.12, respectively and specificity 83.3 %. SsRapid® proved to be a viable, feasibile and cost-effective tool for screening and diagnosing strongyloidiasis subsidizing actions to combat the disease.

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快速免疫层析试验在巴西某流行地区作为圆线虫病诊断潜在工具的评估

尽管将类圆线虫病列为一种被忽视的热带病，并且该病的过度感染和播散形式的死亡率很高，但仍然需要准确的诊断工具。本研究评估了SsRapid®作为一种候选的圆形线虫病快速诊断检测试剂盒，使用的是来自巴西流行地区的特征明确的血清样本。SsRapid®是一种检测IgG4 anti-S的免疫层析测试原型。使用140份来自确诊蛔虫病、其他寄生虫病或健康/无症状患者的样本进行stercoralis，特别是使用重组抗原NIE SsRapid®。计算敏感性（Se）、特异性（Sp）、阳性似然比和阴性似然比（LR+和LR-）的诊断参数。对圆线虫病标本的敏感性为90%，对其他寄生虫病标本的敏感性为17.5%，对健康个体的敏感性为15%。阳性似然比为5.56，阴性似然比为0.12，特异度为83.3%。SsRapid®被证明是一种可行、可行且具有成本效益的筛选和诊断类圆线虫病的工具，并为防治该疾病的行动提供补贴。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.