Plasmonic and quantitative lateral flow assay for grayscale imaging of neutralizing antibodies SARS-CoV-2

IF 3.7 Q1 CHEMISTRY, ANALYTICAL
Karla R. Castro , Isabela A. Mattioli , Graziela C. Sedenho , Thiago Bertaglia , Antônio F.A. Araújo , Manoel J.A. Lima , Beatriz G.R. da Silva , Mona N. Oliveira , Iris Todeschini , Phelipe M. Vitale , Erika R. Manuli , Geovana M. Pereira , Suzete C. Ferreira , Ester C. Sabino , Emanuel Carrilho , Frank N. Crespilho
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引用次数: 0

Abstract

Lateral flow immunoassays (LFIAs) have modernized decentralized mass testing and significantly impacted the healthcare system. It provides a rapid and accessible method for serological testing, which is an important tool for screening patients with past viral phases by detecting antibodies. Here, the development of a quantitative LFIA is presented, in which the receptor-binding domain of Spike protein-specific antibody (anti-S-RBD) in serum samples from previously infected with SARS-CoV-2 patients form an immune complex with the RBD protein immobilized on gold nanoparticles. For quantitative measurements, a methodology was developed based on the plasmonic behavior of gold nanoparticles and using a digital camera that processes images of the test lines in a closed chamber using the CYMK color system. The limits of detection and quantification were 0.22 and 0.70 µg mL–1, respectively. The proposed device has a manufacturing cost of less than US$ 1 from synthesizing the bioconjugates, assembly of the LFIA strips, and 3D printing of the cassettes. The platform could detect anti-S-RBD antibodies in human serum samples and can be particularly useful in strategies for monitoring IgG levels in the population.
中和抗体SARS-CoV-2灰度成像的等离子体和定量横向流动试验
侧流免疫测定法(LFIAs)使分散的大规模检测现代化,并对医疗保健系统产生了重大影响。它提供了一种快速、方便的血清学检测方法,是通过检测抗体筛选过去病毒期患者的重要工具。本文提出了一种定量LFIA的发展,其中先前感染SARS-CoV-2的患者血清样品中的Spike蛋白特异性抗体(抗s -RBD)的受体结合域与固定在金纳米颗粒上的RBD蛋白形成免疫复合物。对于定量测量,开发了一种基于金纳米粒子等离子体行为的方法,并使用数码相机在封闭的腔室中使用CYMK颜色系统处理测试线的图像。检测限为0.22µg mL-1,定量限为0.70µg mL-1。该装置的制造成本低于1美元,包括合成生物偶联物、组装LFIA条带和3D打印卡带。该平台可以检测人血清样本中的抗s - rbd抗体,在监测人群中IgG水平的策略中特别有用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Talanta Open
Talanta Open Chemistry-Analytical Chemistry
CiteScore
5.20
自引率
0.00%
发文量
86
审稿时长
49 days
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