Impact of egg incubation on hemocyte recruitment and susceptibility of Galleria mellonella larvae to pathogens.

IF 4 2区生物学 Q2 MICROBIOLOGY

Frontiers in Microbiology Pub Date : 2025-06-03 eCollection Date: 2025-01-01 DOI:10.3389/fmicb.2025.1611104

Paulo Henrique Fonseca Carmo, Patrícia Michelle Nagai de Lima, Fabiana Alves Silva, Jaqueline Lemes Ribeiro, Juliana Campos Junqueira, Maíra Terra Garcia

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引用次数: 0

Abstract

Introduction: Galleria mellonella is widely used as an in vivo model in microbiological and toxicological studies due to its reproducibility, low cost, and ease of handling. However, maintaining homogeneous laboratory colonies presents challenges. This study evaluated whether different incubation methods influence the larval immune response against Staphylococcus aureus, Candida albicans, and Escherichia coli.

Methods: Four experimental groups were established: (1) Traditional group (TG), eggs stored at 27°C; (2) Immediate group (IG), eggs stored at 16°C for 120 days before incubation at 27°C; (3) Gradual group (GG), temperature gradually reduced from 27°C to 16°C over 98 days, then increased back; and (4) Frozen group (FG), eggs stored at freezing temperatures.

Results and discussion: FG eggs were non-viable, and IG and GG showed delayed development. IG larvae were more susceptible to infections, while GG larvae resembled TG. Thus, GG can serve as an effective alternative rearing method.

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卵孵育对成熟Galleria mellonella幼虫血细胞募集及对病原体敏感性的影响。

mellonella因其可重复性好、成本低、易于处理等优点，被广泛用作微生物学和毒理学研究的体内模型。然而，保持同质的实验室菌落存在挑战。本研究评估了不同的孵育方法是否会影响幼虫对金黄色葡萄球菌、白色念珠菌和大肠杆菌的免疫反应。方法：设4个实验组：(1)传统组（TG），鸡蛋27℃保存；(2)直接组（IG）， 16℃保存120天，27℃孵育；(3)渐进式组（GG）， 98 d内温度由27℃逐渐降至16℃，而后回升；(4)冷冻组（FG），在冷冻温度下储存的鸡蛋。结果与讨论：FG卵不能活，IG和GG发育迟缓。IG幼虫易受感染，而GG幼虫与TG相似。因此，GG可以作为一种有效的替代饲养方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Microbiology MICROBIOLOGY-

CiteScore

7.70

自引率

9.60%

发文量

4837

审稿时长

14 weeks

期刊介绍： Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.