MiR-106b-5p, MiR-200c-3p, and MiR-146a-5p expression as putative biomarkers for disease state in primary immune thrombocytopenia.

IF 2.1 4区医学 Q2 HEMATOLOGY

Expert Review of Hematology Pub Date : 2025-06-25 DOI:10.1080/17474086.2025.2522298

Reham Salah El Zaiat, Mai A H Abouelenin, Amany A Saleh, Mahmoud El-Hawy, Iman Aly Ahmedy, Manal Monir Mansour

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引用次数: 0

Abstract

Background: The pathophysiology of primary immune thrombocytopenia (ITP) is complicated and multifactorial, including platelet antibody formation and T cell imbalance. Emerging evidence has revealed differential miRNA expression in autoimmune disorders, including ITP. Nevertheless, the role of miR-106b-5p, miR-200c-3p, and miR-146a-5p in ITP remains unclear. Herein, we explored the potential role of these miRNAs in pediatric ITP and examined how their plasma levels influenced response to therapy.

Research design and methods: Three groups were recruited in this study: newly diagnosed ITP children (n = 25) in group I, chronic ITP children (n = 25) in group II, and normal controls (n = 25) in group III. Plasma levels of miR-106p-5p, miR-200c-3p, and miR-146a-5p were measured by polymerase chain reaction.

Results: MiR-106b-5p and miR-200c-3p were upregulated, whereas miR-146a-5p was downregulated in newly diagnosed and chronic ITP versus controls. MiR-200c-3p and miR-146a-5p were much higher in chronic ITP than newly diagnosed ITP. Lower miR-106b-5p levels were associated with complete response.

Conclusions: MiR-106b-5p and miR-200c-3p were elevated, while miR-146a-5p was suppressed in ITP versus controls. Reduced miR-106b-5p indicated a full response to therapy. These markers may be useful as diagnostic ITP biomarkers. Moreover, miR-106b-5p level can be used to monitor response to therapy and as a predictor for complete response.

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MiR-106b-5p， MiR-200c-3p和MiR-146a-5p表达作为原发性免疫性血小板减少症疾病状态的推定生物标志物。

背景：原发性免疫性血小板减少症（ITP）的病理生理是复杂的、多因素的，包括血小板抗体形成和T细胞失衡。新出现的证据揭示了自身免疫性疾病（包括ITP）中miRNA的差异表达。然而，miR-106b-5p、miR-200c-3p和miR-146a-5p在ITP中的作用尚不清楚。在此，我们探讨了这些mirna在儿科ITP中的潜在作用，并检查了它们的血浆水平如何影响对治疗的反应。研究设计与方法：本研究分为三组，ⅰ组为新诊断ITP患儿（n = 25），ⅱ组为慢性ITP患儿（n = 25），ⅲ组为正常对照组（n = 25）。采用聚合酶链反应检测miR-106p-5p、miR-200c-3p和miR-146a-5p的血浆水平。结果：与对照组相比，MiR-106b-5p和miR-200c-3p在新诊断和慢性ITP中上调，而miR-146a-5p下调。MiR-200c-3p和miR-146a-5p在慢性ITP中的表达明显高于新诊断的ITP。miR-106b-5p水平较低与完全缓解相关。结论：与对照组相比，ITP中MiR-106b-5p和miR-200c-3p升高，而miR-146a-5p被抑制。miR-106b-5p降低表明对治疗有完全反应。这些标记物可作为ITP的诊断性生物标记物。此外，miR-106b-5p水平可用于监测对治疗的反应，并作为完全反应的预测指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Expert Review of Hematology HEMATOLOGY-

CiteScore

4.70

自引率

3.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Advanced molecular research techniques have transformed hematology in recent years. With improved understanding of hematologic diseases, we now have the opportunity to research and evaluate new biological therapies, new drugs and drug combinations, new treatment schedules and novel approaches including stem cell transplantation. We can also expect proteomics, molecular genetics and biomarker research to facilitate new diagnostic approaches and the identification of appropriate therapies. Further advances in our knowledge regarding the formation and function of blood cells and blood-forming tissues should ensue, and it will be a major challenge for hematologists to adopt these new paradigms and develop integrated strategies to define the best possible patient care. Expert Review of Hematology (1747-4086) puts these advances in context and explores how they will translate directly into clinical practice.