Investigating the Efficacy of Amoxicillin-Broccoli Combination in Combating Multidrug-Resistant Staphylococcus aureus Infection.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2025-06-18 DOI:10.1007/s00284-025-04316-9

Habiba Mosaad, Engy Elekhnawy, Walaa A Negm, Salwa S Younis, Mona El-Aasr

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Abstract

In light of the propagation of resistance amongst bacteria, many infections are currently difficult to treat, with extremely high death and morbidity rates. Thus, we investigated the effect of using a combination of amoxicillin and broccoli ethanolic extract against Staphylococcus aureus infections. Broccoli or Brassica oleracea active metabolites' were to be identified and quantified using high-performance liquid chromatography (HPLC). The major detected compounds were chlorogenic acid and gallic acid. In vitro, the combination of amoxicillin with broccoli revealed fractional inhibitory concentration index of the amoxicillin-broccoli combination was less than 0.5 in 50% of the isolates, which indicates a synergetic potential. The in vivo study revealed a notable lessening in the bacterial count present in the examined tissues in the employed systemic infection model. The studied tissues were kidney, liver, and spleen. Moreover, the histological characteristics were found to have a remarkable enhancement in the combination-treated group. Thus, future clinical reports have to be employed to explicate the usefulness of such a combination in the clinical practice.

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阿莫西林-西兰花联合治疗耐多药金黄色葡萄球菌感染的疗效研究。

鉴于细菌之间耐药性的传播，许多感染目前难以治疗，死亡率和发病率极高。因此，我们研究了阿莫西林和西兰花乙醇提取物联合使用对金黄色葡萄球菌感染的影响。采用高效液相色谱法（HPLC）对西兰花或甘蓝的活性代谢物进行鉴定和定量。检测到的主要化合物为绿原酸和没食子酸。体外实验结果表明，阿莫西林-西兰花联合抑菌浓度指数在50%以内，具有一定的协同效应。体内研究显示，在所采用的全身性感染模型中，在所检查的组织中存在的细菌计数显着减少。研究组织为肾、肝、脾。此外，联合治疗组的组织学特征明显增强。因此，未来的临床报告必须用来说明这种组合在临床实践中的有用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.