Progesterone-controlled internal drug release-based estrous synchronization induces vaginal dysbiosis and enrichment of potential pathogens in Braford heifers.
Candelaria Gonzalez Moreno, Solana Funiciello-Mendez, Andrea Torres Luque, Pavly Fayek, Aspinas Chapwanya, María C Otero
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引用次数: 0
Abstract
Objective: To evaluate the effects of an estrus synchronization program on the resident microbial population of the vagina in Braford heifers.
Methods: Nulliparous Braford heifers were enrolled in a 9-day progesterone-controlled internal drug release-based estrous synchronization protocol. Vaginal swabs were collected on day (D) 0 and D11 of the synchronization protocol and again on D60 to profile changes in vaginal bacterial communities though 16S ribosomal DNA gene sequencing.
Results: Analysis of samples from 10 heifers revealed that the most abundant phyla were the Proteobacteria, Firmicutes, Bacteroidetes, Tenericutes, Fusobacteria, and Actinobacteria. Principal coordinate analysis showed significant differentiation among the vaginal profiles of the microbial communities at D11 compared to D0 and D60. The bacterial clustering may be due to estrous synchronization. Significant differences were observed at the genus level. The relative abundances of known endometrial pathogens Fusobacterium, Helcococcus, Ureaplasma, and Leptotrichiaceae significantly increased from D0 to D11.
Conclusions: This study describes, for the first time, transient changes in the diversity of the vaginal microbiota of Braford heifers in response to estrus manipulations. The presence (or absence) of certain recognized pathogens, such as Fusobacterium, Helcococcus, and Ureaplasma, may negatively affect reproductive health, justifying further investigation.
Clinical relevance: These results may help in advances of the diagnosis and treatment of infertility.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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