Combined cellulose acetate butyrate and palmitic acid solvent exchange-driven phase inversion in situ gel loading moxifloxacin HCl for periodontitis therapy

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2025-06-16 DOI:10.1016/j.ijbiomac.2025.145314

Warakon Thammasut , Wantanwa Krongrawa , Catleya Rojviriya , Pisit Pitchayakorn , Thawatchai Phaechamud

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Abstract

This study develops in situ forming gels (ISGs) incorporating cellulose acetate butyrate (CAB) and palmitic acid (PAL) as matrix-forming agents for localized periodontal drug delivery. The aim is to investigate the effects of CAB, PAL, and their combination on viscosity, rheological behavior, matrix formation, drug release, structural properties, and antimicrobial efficacy of moxifloxacin (Mx)-loaded ISGs. Drug release was examined, and release kinetics were determined using mathematical modeling. Scanning electron microscopy (SEM) and X-ray tomographic microscopy (XTM) were used to characterize matrix structures. Antimicrobial activity was assessed against Staphylococcus aureus, Candida albicans, and Porphyromonas gingivalis over 14 days using an agar diffusion assay. CAB dominant ISGs formed dense, slow-releasing matrices, while PAL dominant ISGs exhibited rapid phase inversion due to swift solvent exchange and higher porosity. NMP-based ISGs formed more porous matrices than DMSO-based ISGs in PAL-dominant formulations, enhancing drug diffusion at later stages. CAB/PAL combination ISGs balanced injectability, phase inversion, and prolonged drug release. Antimicrobial studies also confirmed sustained activity, with CAB dominant formulations maintaining efficacy for 14 days. CAB/PAL-based ISGs offer a promising strategy for sustained periodontal drug delivery while solvent selection modulates porosity and antimicrobial activity. This system demonstrates potential for prolonged therapeutic efficacy in localized periodontal treatment.

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醋酸丁酸纤维素与棕榈酸溶剂交换驱动相转化原位凝胶负载盐酸莫西沙星治疗牙周炎

本研究开发了含有醋酸丁酸纤维素（CAB）和棕榈酸（PAL）作为基质形成剂的原位形成凝胶（ISGs），用于局部牙周药物输送。目的是研究CAB、PAL及其组合对莫西沙星（Mx）负载isg的粘度、流变行为、基质形成、药物释放、结构特性和抗菌功效的影响。检测药物释放，并利用数学模型确定释放动力学。采用扫描电子显微镜（SEM）和x射线层析显微镜（XTM）对基体结构进行表征。采用琼脂扩散法对金黄色葡萄球菌、白色念珠菌和牙龈卟啉单胞菌的抗菌活性进行了14天的评估。以CAB为主的isg形成致密的缓释基质，而以PAL为主的isg由于溶剂交换迅速和孔隙率高，表现出快速的相转化。在以pal为主的配方中，NMP-based isg比DMSO-based isg形成了更多的多孔基质，从而增强了药物在后期的扩散。CAB/PAL联合isg平衡了可注射性、相转化和延长药物释放。抗菌研究也证实了持续的活性，CAB主导配方保持药效14天。基于CAB/ pal的isg为牙周药物持续递送提供了有前途的策略，而溶剂选择调节孔隙度和抗菌活性。该系统在局部牙周治疗中具有延长治疗效果的潜力。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.