Full genome characterization of a Kenyan G8P[14] rotavirus strain suggests artiodactyl-to-human zoonotic transmission.

IF 3.6 Q1 TROPICAL MEDICINE

Tropical Medicine and Health Pub Date : 2025-06-16 DOI:10.1186/s41182-025-00759-9

Ernest Apondi Wandera, Yuki Akari, Carlene Sang, Pamela Njugu, Samoel Ashimosi Khamadi, Sebastian Musundi, Maurine Mumo Mutua, Saori Fukuda, Takayuki Murata, Shingo Inoue, Satoshi Kaneko, James Nyangao, Satoshi Komoto

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Abstract

Background: Rotavirus infections are a major cause of severe gastroenteritis in children. Human rotavirus strains with the unconventional G8P[14] genotype have sporadically been detected in diarrheic patients in different parts of the world. However, full genomes of only two human G8P[14] strains from Africa (North Africa) have been sequenced, and the origin and evolutionary patterns of African G8P[14] strains remain to be elucidated.

Methods: In this study, we sequenced the full genome of an African G8P[14] strain (RVA/Human-wt/KEN/A75/2000/G8P[14]) identified in archival stool samples from a diarrheic child in Kenya.

Results: Full genome-based analysis of strain A75 revealed a unique genogroup constellation, G8-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3, with the I2-R2-C2-M2-A11-N2-T6-E2-H3 part being common among rotavirus strains from artiodactyls such as cattle. Phylogenetic analysis showed that all the 11 genomic segments of strain A75 are closely related to segments found in artiodactyl rotavirus strains, and likely strain A75 derived from spillover transmission of an artiodactyl rotavirus strain to humans.

Conclusion: This is the first report on a full genome-based characterization of a human G8P[14] strain from East Africa. This study demonstrates the diversity of human G8P[14] strains in Africa and contributes to the elucidation of their spreading and evolution, which includes zoonotic transmission from artiodactyls.

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肯尼亚G8P[14]轮状病毒株的全基因组特征表明，偶蹄动物与人类的人畜共患传播。

背景：轮状病毒感染是儿童严重肠胃炎的主要原因。具有非常规G8P[14]基因型的人轮状病毒株已在世界不同地区的腹泻患者中零星发现。然而，仅有两株来自非洲（北非）的人类G8P[14]菌株的全基因组测序完成，非洲G8P[14]菌株的起源和进化模式仍有待阐明。方法：在本研究中，我们对一株非洲G8P[14]菌株（RVA/Human-wt/KEN/A75/2000/G8P[14]）进行了全基因组测序，该菌株来自肯尼亚一名腹泻儿童的档案粪便样本。结果：对A75株进行全基因组分析，发现其独特的基因群群为G8-P[14]-I2-R2-C2-M2-A11-N2-T6-E2-H3，其中I2-R2-C2-M2-A11-N2-T6-E2-H3部分在牛等偶蹄动物轮状病毒株中普遍存在。系统发育分析表明，菌株A75的11个基因组片段均与偶蹄轮状病毒毒株中发现的片段密切相关，菌株A75可能来源于偶蹄轮状病毒毒株对人类的外泄传播。结论：本文首次报道了一株来自东非的人类G8P[14]菌株的全基因组特征。本研究证明了非洲人类G8P[14]菌株的多样性，并有助于阐明其传播和进化，其中包括偶蹄动物的人畜共患传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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