Effects of Experimental Periodontitis and Periodontal Treatment on Alzheimer-Like Pathology in Rats.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-06-16 DOI:10.1111/odi.70001

Sedanur Yavuz, Birsen Elibol, Esra Demir, Beyza Kinsiz, Ozlem Toluk, Sadiye Gunpinar

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引用次数: 0

Abstract

Objective: This study investigated the effects of periodontitis (P) and non-surgical periodontal therapy (NSPT) on behavior, neurodegeneration, and neuroinflammation in rats with Alzheimer's disease (AD)-like pathology.

Methods: AD-like pathology was induced in rats (n = 28) using STZ neurodegeneration model. Periodontitis was experimentally induced (n = 32), and half of which received NSPT with Chlorhexidine (CHX) gel. Behavioral assessment included the passive avoidance task (PA) and Morris water maze (MWM). Levels of NLRP3, phosphorylated tau (p-tau), and tau in the hippocampus, cerebrospinal fluid (CSF), and serum were measured by ELISA, while BACE1, IL1β, iNOS, and NF-κβ proteins were assessed by Western blotting.

Results: Rats in the AD and AD + P groups performed worse in behavioral tests compared to controls (p < 0.05), whereas the NSPT group showed similar performance to controls (p > 0.05). CSF p-tau levels were comparable between AD and AD + P groups, but the hippocampal p-tau/tau ratio was significantly higher in the AD + P group (p < 0.05). BACE1 levels were similar in P and AD groups. NLRP3 and iNOS levels did not show significant differences across groups. Notably, the NSPT group exhibited reduced NF-κβ levels (p < 0.05).

Conclusions: Periodontitis may exacerbate AD-like molecular pathology, particularly by promoting tau hyperphosphorylation, while NSPT appears to mitigate disease progression and improve behavioral outcomes.

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实验性牙周炎及牙周治疗对大鼠阿尔茨海默样病理的影响。

目的：探讨牙周炎(P)和非手术牙周治疗（NSPT）对阿尔茨海默病（AD）样病理大鼠行为、神经变性和神经炎症的影响。方法：采用STZ神经退行性变模型对28只大鼠进行ad样病理诱导。实验诱导牙周炎32例，其中一半采用氯己定凝胶NSPT治疗。行为评估包括被动回避任务（PA）和Morris水迷宫（MWM）。ELISA法检测海马、脑脊液和血清中NLRP3、磷酸化tau （p-tau）和tau蛋白水平，Western blotting法检测BACE1、il - 1β、iNOS和NF-κβ蛋白水平。结果：AD组和AD + P组大鼠在行为测试中的表现较对照组差（P < 0.05）。AD组和AD + P组脑脊液P -tau水平相当，但AD + P组海马P -tau/tau比值显著高于AD + P组(P结论：牙周炎可能加剧AD样分子病理，特别是通过促进tau过度磷酸化，而NSPT似乎可以缓解疾病进展并改善行为结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.