PPDO-induced tunable degradation and HA-enhanced osteogenesis in PLCL scaffolds for bone regeneration.

IF 3.6 4区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of Biomaterials Science, Polymer Edition Pub Date : 2025-06-16 DOI:10.1080/09205063.2025.2519868

Jianfei Cao, Chao Peng, Yan Lei, Haoming Wu, Shaojuan Xu, Qiyu Liu, Yi Liu, Mengjue Li, Yue Lu

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引用次数: 0

Abstract

Poly(lacticacid-ε-caprolactone) (PLCL) scaffolds face significant challenges in bone regeneration due to excessively slow degradation kinetics and inherent hydrophobicity. To overcome these limitations, we developed a novel ternary 3D-printed scaffold composed of PLCL, poly(p-dioxanone) (PPDO), and hydroxyapatite (HA) via fused deposition modeling (FDM) for the first time. The incorporation of PPDO would accelerate and enable tunable degradation of PLCL to match the bone healing timeline, while HA was aimed to enhance osteoinductivity and regulated the pH level to reduce adverse immune reactions of the acidic degradation products. The results demonstrated that degradation rate of the scaffolds was found to be modulated by PPDO and HA effectively. Moreover, the 3D printing extrusion enabled the porous scaffolds with customizability, diverse shapes, adjustable porosity and uniform pore sizes. In addition, proliferation and adhesion of bone marrow mesenchymal stem cells (BMSCs) as well as the expression of various osteogenic genes (ALP, Col-Ι, OCN, BMP-2, OPN) were also upregulated on the PLCL/PPDO/HA scaffolds. Therefore, these low-cost 3D-printed scaffolds may serve as an optimal bone graft for applications in bone tissue engineering.

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ppdo诱导的可调节降解和ha增强PLCL支架骨再生的成骨作用。

聚乳酸-ε-己内酯（PLCL）支架由于降解动力学过慢和固有的疏水性，在骨再生方面面临重大挑战。为了克服这些限制，我们首次通过熔融沉积建模（FDM）开发了一种由PLCL、聚对二氧环酮（PPDO）和羟基磷灰石（HA）组成的新型三元3d打印支架。加入PPDO可以加速PLCL的可调节降解，使其与骨愈合时间相匹配，而HA旨在增强骨诱导能力，调节pH水平，以减少酸性降解产物的不良免疫反应。结果表明，PPDO和HA可有效调节支架的降解速率。此外，3D打印挤压使多孔支架具有可定制性、形状多样、孔隙度可调、孔径均匀等特点。此外，骨髓间充质干细胞（BMSCs）的增殖和粘附以及各种成骨基因（ALP、Col-Ι、OCN、BMP-2、OPN）的表达也在PLCL/PPDO/HA支架上上调。因此，这些低成本的3d打印支架可以作为骨组织工程应用的最佳骨移植物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biomaterials Science, Polymer Edition 工程技术-材料科学：生物材料

CiteScore

7.10

自引率

5.60%

发文量

117

审稿时长

1.5 months

期刊介绍： The Journal of Biomaterials Science, Polymer Edition publishes fundamental research on the properties of polymeric biomaterials and the mechanisms of interaction between such biomaterials and living organisms, with special emphasis on the molecular and cellular levels. The scope of the journal includes polymers for drug delivery, tissue engineering, large molecules in living organisms like DNA, proteins and more. As such, the Journal of Biomaterials Science, Polymer Edition combines biomaterials applications in biomedical, pharmaceutical and biological fields.