Investigation of the Protective Effect of Red-Light-Intervened Dendrobium officinale on Rats With Gastric Injury Based on Serum Metabolomics

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-06-18 DOI:10.1002/bmc.70135

Huimin Luo, Xuejing Dong, Long Chen, Fengfeng Xie, Liba Xu, Miao Zhang, Hua Zhu, Jianbei Teng, Zhonghua Dai, Xiuqi Yu

{"title":"Investigation of the Protective Effect of Red-Light-Intervened Dendrobium officinale on Rats With Gastric Injury Based on Serum Metabolomics","authors":"Huimin Luo, Xuejing Dong, Long Chen, Fengfeng Xie, Liba Xu, Miao Zhang, Hua Zhu, Jianbei Teng, Zhonghua Dai, Xiuqi Yu","doi":"10.1002/bmc.70135","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>This study aimed to compare both the protective effects and metabolic mechanisms of red-light-cultured <i>Dendrobium officinale</i> (RL-DO) and natural-light-cultured <i>D. officinale</i> (NL-DO) on gastric injury in rats. Rats were divided into control, model, omeprazole, RL-DO, and NL-DO groups. After 24-day treatment, gastric mucosal pathology, serum biomarkers (Gas, MTL, and SS), oxidative stress markers (SOD, MDA, and NO), apoptosis-related proteins (Bax/Bcl-2 and Caspase-3), and serum metabolomics were analyzed via HE staining, ELISA, Western blot, TUNEL, and UHPLC-Q-Exactive Plus-MS. Both RL-DO and NL-DO significantly improved gastric mucosal damage, upregulated SOD/NO, downregulated MDA, reduced apoptosis, and modulated Bax/Bcl-2/Caspase-3 (<i>p</i> < 0.05/0.01). RL-DO exhibited superior efficacy in enhancing body weight, water intake, fecal moisture, and NO levels. Metabolomics revealed RL-DO reversed 46 metabolites (vs. NL-DO's 37) and targeted key pathways including glycerophospholipid metabolism, apoptosis regulation, and cAMP signaling. RL-DO uniquely regulated arginine/proline metabolism and fatty acid biosynthesis. Both RL-DO and NL-DO protect against gastric injury, with RL-DO showing enhanced efficacy, likely due to broader metabolic pathway modulation.</p>\n </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 8","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Chromatography","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bmc.70135","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

This study aimed to compare both the protective effects and metabolic mechanisms of red-light-cultured Dendrobium officinale (RL-DO) and natural-light-cultured D. officinale (NL-DO) on gastric injury in rats. Rats were divided into control, model, omeprazole, RL-DO, and NL-DO groups. After 24-day treatment, gastric mucosal pathology, serum biomarkers (Gas, MTL, and SS), oxidative stress markers (SOD, MDA, and NO), apoptosis-related proteins (Bax/Bcl-2 and Caspase-3), and serum metabolomics were analyzed via HE staining, ELISA, Western blot, TUNEL, and UHPLC-Q-Exactive Plus-MS. Both RL-DO and NL-DO significantly improved gastric mucosal damage, upregulated SOD/NO, downregulated MDA, reduced apoptosis, and modulated Bax/Bcl-2/Caspase-3 (p < 0.05/0.01). RL-DO exhibited superior efficacy in enhancing body weight, water intake, fecal moisture, and NO levels. Metabolomics revealed RL-DO reversed 46 metabolites (vs. NL-DO's 37) and targeted key pathways including glycerophospholipid metabolism, apoptosis regulation, and cAMP signaling. RL-DO uniquely regulated arginine/proline metabolism and fatty acid biosynthesis. Both RL-DO and NL-DO protect against gastric injury, with RL-DO showing enhanced efficacy, likely due to broader metabolic pathway modulation.

查看原文本刊更多论文

基于血清代谢组学研究红光干预铁皮石斛对胃损伤大鼠的保护作用

本研究旨在比较红光培养铁皮石斛（RL-DO）和自然光培养铁皮石斛（NL-DO）对大鼠胃损伤的保护作用和代谢机制。将大鼠分为对照组、模型组、奥美拉唑组、RL-DO组和NL-DO组。治疗24天后，通过HE染色、ELISA、Western blot、TUNEL和UHPLC-Q-Exactive - ms分析胃粘膜病理、血清生物标志物（Gas、MTL和SS）、氧化应激标志物（SOD、MDA和NO）、凋亡相关蛋白（Bax/Bcl-2和Caspase-3）和血清代谢组学。RL-DO和NL-DO均能显著改善胃黏膜损伤，上调SOD/NO，下调MDA，减少细胞凋亡，调节Bax/Bcl-2/Caspase-3 （p < 0.05/0.01）。RL-DO在提高体重、饮水量、粪便水分和一氧化氮水平方面表现出优异的效果。代谢组学显示，RL-DO逆转了46种代谢物（NL-DO逆转了37种），并靶向了包括甘油磷脂代谢、细胞凋亡调节和cAMP信号传导在内的关键途径。RL-DO独特地调节精氨酸/脯氨酸代谢和脂肪酸生物合成。RL-DO和NL-DO都能预防胃损伤，其中RL-DO的效果更强，可能是由于更广泛的代谢途径调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.