Targeted next-generation sequencing reveals hemotropic mycoplasmas, Bartonella spp., and Babesia in shelter dogs from Texas, USA

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-06-15 DOI:10.1016/j.vprsr.2025.101297

Ian Kirimi Daniel , Maureen A. Kelly , Emily Orr , Hassan Hakimi , Rafael A.N. Ramos , Meriam N. Saleh , Jobin J. Kattoor , Rebecca P. Wilkes , Guilherme G. Verocai

{"title":"Targeted next-generation sequencing reveals hemotropic mycoplasmas, Bartonella spp., and Babesia in shelter dogs from Texas, USA","authors":"Ian Kirimi Daniel , Maureen A. Kelly , Emily Orr , Hassan Hakimi , Rafael A.N. Ramos , Meriam N. Saleh , Jobin J. Kattoor , Rebecca P. Wilkes , Guilherme G. Verocai","doi":"10.1016/j.vprsr.2025.101297","DOIUrl":null,"url":null,"abstract":"<div><div>The screening of vector-borne pathogens (VBPs) in shelter dog populations is crucial for early detection and informs proper management and control efforts. In this study, we used a targeted next-generation sequencing (tNGS) assay to detect VBPs in shelter dogs from Texas. Genomic DNA from whole blood (n = 153) was extracted and subjected to the tNGS assay to search for 15 pathogens. A total of 26.1 % (n = 40/153) of dogs were positive for at least one pathogen, with 80 % (32/40) and 20 % (8/40) samples positive for single and co-infections, respectively. Considering single infections, Mycoplasma haemocanis (13.0 %; n = 20/153) was the most detected pathogen, followed by Bartonella vinsonii subsp. berkhoffii (5.8 %; n = 9/153), and Candidatus Mycoplasma haematoparvum (1.9 %; n = 3/153). Among co-infections, Cand. M. haematoparvum + M. haemocanis (2.6 %; n = 4/153), followed by B. v. berkhoffii + M. haemocanis (1.3 %; n = 2/153), B. v. berkhoffii + Cand. M. haematoparvum (0.7 %; n = 1/153) and B. vulpes + M. haemocanis (0.7 %; n = 1/153). We did not find any association between demographic variables (e.g., age, sex, spayed or neutered status, and breed) and pathogen detection. This study underscores the importance of hemotropic mycoplasma and co-infecting pathogen surveillance in shelter dogs and emphasizes the need for robust and comprehensive assays that allow the simultaneous detection of multiple canine VBPs. Additionally, based on the detection of some pathogens with zoonotic potential, prevention strategies, including comprehensive screenings and targeted biosecurity measures, are recommended for minimizing the risk of exposure to their future owners.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101297"},"PeriodicalIF":1.4000,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology, regional studies and reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405939025001054","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The screening of vector-borne pathogens (VBPs) in shelter dog populations is crucial for early detection and informs proper management and control efforts. In this study, we used a targeted next-generation sequencing (tNGS) assay to detect VBPs in shelter dogs from Texas. Genomic DNA from whole blood (n = 153) was extracted and subjected to the tNGS assay to search for 15 pathogens. A total of 26.1 % (n = 40/153) of dogs were positive for at least one pathogen, with 80 % (32/40) and 20 % (8/40) samples positive for single and co-infections, respectively. Considering single infections, Mycoplasma haemocanis (13.0 %; n = 20/153) was the most detected pathogen, followed by Bartonella vinsonii subsp. berkhoffii (5.8 %; n = 9/153), and Candidatus Mycoplasma haematoparvum (1.9 %; n = 3/153). Among co-infections, Cand. M. haematoparvum + M. haemocanis (2.6 %; n = 4/153), followed by B. v. berkhoffii + M. haemocanis (1.3 %; n = 2/153), B. v. berkhoffii + Cand. M. haematoparvum (0.7 %; n = 1/153) and B. vulpes + M. haemocanis (0.7 %; n = 1/153). We did not find any association between demographic variables (e.g., age, sex, spayed or neutered status, and breed) and pathogen detection. This study underscores the importance of hemotropic mycoplasma and co-infecting pathogen surveillance in shelter dogs and emphasizes the need for robust and comprehensive assays that allow the simultaneous detection of multiple canine VBPs. Additionally, based on the detection of some pathogens with zoonotic potential, prevention strategies, including comprehensive screenings and targeted biosecurity measures, are recommended for minimizing the risk of exposure to their future owners.

查看原文本刊更多论文

靶向下一代测序揭示了美国德克萨斯州收容所狗中的嗜血性支原体，巴尔通体和巴贝斯虫

在收容犬群中筛查媒介传播病原体（VBPs）对于早期发现至关重要，并为适当的管理和控制工作提供信息。在这项研究中，我们使用了一种靶向下一代测序（tNGS）方法来检测来自德克萨斯州收容所的狗的VBPs。提取全血基因组DNA (n = 153)，进行tNGS检测，寻找15种病原体。26.1% （n = 40/153）的狗至少有一种病原体呈阳性，其中80%（32/40）的样本呈阳性，20%（8/40）的样本呈阳性。考虑到单次感染，血液支原体（13.0%）；n = 20/153)是检出最多的病原菌，其次是文氏巴尔通体亚种。Berkhoffii (5.8%；n = 9/153)，候选血支原体(1.9%；n = 3/153)。在合并感染中，Cand。血猪支原体+血猪支原体(2.6%；n = 4/153)，其次是伯克氏芽孢杆菌+血红芽孢杆菌(1.3%；n = 2/153)， B. v. berkhoffii + c。血疟原虫(0.7%；n = 1/153)和vulpes + haemocanis (0.7%；n = 1/153)。我们没有发现人口统计变量（如年龄、性别、绝育状态和品种）与病原体检测之间的任何关联。本研究强调了对收容犬进行嗜血性支原体和共感染病原体监测的重要性，并强调了对多种犬vbp同时检测的强大和全面的分析的必要性。此外，根据对一些具有人畜共患潜力的病原体的检测，建议采取预防策略，包括全面筛查和有针对性的生物安全措施，以尽量减少其未来所有者接触的风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).