Gelatin-guar gum hydrogel for topical application: cytotoxicity, antibacterial activity against MRSA, and non-irritant characteristics

IF 5.8 2区化学 Q1 POLYMER SCIENCE

European Polymer Journal Pub Date : 2025-06-12 DOI:10.1016/j.eurpolymj.2025.114059

Francisca Vanessa C. Canafístula , Matheus X. Oliveira , Ana Jérsia Araújo , José Delano B. Marinho Filho , Rodrigo Elísio de Sá , Alyne R. Araujo-Nobre , Sansara Sanny M. Araújo , Fábio Oliveira S. Ribeiro , Roberta Jeane B. Jorge , Ana Celeste X. Oliveira , Fpelipe Rhaynan S. Andrade , Jeanny S. Maciel , Regina Celia M. de Paula , Judith Pessoa A. Feitosa

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引用次数: 0

Abstract

Hydrogels based on oxidized guar gum (OxGG) and gelatin (Gel) were synthesized via Schiff base crosslinking using three different gelatin proportions (60–80 wt%). The hydrogels were characterized by rheology, FT-IR, GPC, SEM, swelling behavior, and degradation analyses. Variations in gelatin content led to hydrogels with distinct physicochemical properties; higher gelatin concentrations resulted in shorter gelation times, increased crosslinking density, lower swelling capacity, smaller interconnected porous structures, and faster degradation in PBS. All three hydrogels exhibited strong intrinsic antibacterial activity, inhibiting methicillin/oxacillin-resistant Staphylococcus aureus (MRSA) by >98 % and Staphylococcus epidermidis and Staphylococcus aureus by >95 %. The performance of these hydrogels was compared with that of other recently reported gels exhibiting high MRSA inhibition as well as with gelatin-containing hydrogels. Notably, OxGG:Gel hydrogels stood out because of their high antibacterial activity, which was achieved without the addition of antibiotic agents or the need for additional treatments such as photothermal therapy. Furthermore, all hydrogels demonstrated good cell viability and did not induce significant changes in the CAM blood vessels, such as vascular lysis, hemorrhage, or coagulation, confirming their non-irritating nature. The OxGG:Gel70 hydrogel exhibited superior cell viability, intermediate degradation rate, and strong efficacy against MRSA. In addition to being nonirritating, it exhibited a protective effect against vascular events. Moreover, this hydrogel exhibited key functional properties, including injectability, self-healing, and adhesiveness. Given these characteristics, OxGG:Gel70 stands out among MRSA-active hydrogels and has great potential for topical applications, including dermatitis, skin inflammation, ophthalmic formulations, and wound healing.

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局部应用的明胶瓜尔胶水凝胶：细胞毒性，对MRSA的抗菌活性，和非刺激性特性

以氧化瓜尔胶（OxGG）和明胶（Gel）为基础，采用三种不同的明胶比例（60-80 wt%），通过希夫碱交联合成了水凝胶。通过流变学、FT-IR、GPC、SEM、膨胀行为和降解分析对水凝胶进行了表征。明胶含量的变化导致水凝胶具有不同的物理化学性质；较高的明胶浓度导致胶凝时间缩短，交联密度增加，溶胀能力降低，相互连接的多孔结构更小，在PBS中降解更快。这三种水凝胶均表现出较强的内在抗菌活性，对耐甲氧西林/氧西林金黄色葡萄球菌（MRSA）的抑制率为98%，对表皮葡萄球菌和金黄色葡萄球菌的抑制率为95%。这些水凝胶的性能与最近报道的其他具有高MRSA抑制作用的凝胶以及含有明胶的水凝胶进行了比较。值得注意的是，OxGG:Gel水凝胶因其高抗菌活性而脱颖而出，而不需要添加抗生素或光热疗法等额外治疗。此外，所有水凝胶均表现出良好的细胞活力，不会引起CAM血管的明显变化，如血管溶解、出血或凝血，证实了它们的无刺激性。OxGG:Gel70水凝胶表现出优异的细胞活力、中等降解率和对MRSA的强效。除了无刺激性外，它还表现出对血管事件的保护作用。此外，这种水凝胶表现出关键的功能特性，包括可注射性、自愈性和粘附性。鉴于这些特点，OxGG:Gel70在mrsa活性水凝胶中脱颖而出，具有很大的局部应用潜力，包括皮炎，皮肤炎症，眼科配方和伤口愈合。

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来源期刊

European Polymer Journal 化学-高分子科学

CiteScore

9.90

自引率

10.00%

发文量

691

审稿时长

23 days

期刊介绍： European Polymer Journal is dedicated to publishing work on fundamental and applied polymer chemistry and macromolecular materials. The journal covers all aspects of polymer synthesis, including polymerization mechanisms and chemical functional transformations, with a focus on novel polymers and the relationships between molecular structure and polymer properties. In addition, we welcome submissions on bio-based or renewable polymers, stimuli-responsive systems and polymer bio-hybrids. European Polymer Journal also publishes research on the biomedical application of polymers, including drug delivery and regenerative medicine. The main scope is covered but not limited to the following core research areas: Polymer synthesis and functionalization • Novel synthetic routes for polymerization, functional modification, controlled/living polymerization and precision polymers. Stimuli-responsive polymers • Including shape memory and self-healing polymers. Supramolecular polymers and self-assembly • Molecular recognition and higher order polymer structures. Renewable and sustainable polymers • Bio-based, biodegradable and anti-microbial polymers and polymeric bio-nanocomposites. Polymers at interfaces and surfaces • Chemistry and engineering of surfaces with biological relevance, including patterning, antifouling polymers and polymers for membrane applications. Biomedical applications and nanomedicine • Polymers for regenerative medicine, drug delivery molecular release and gene therapy The scope of European Polymer Journal no longer includes Polymer Physics.