Metabolite profiling and cytotoxicity of Tragopogon dubius fractions against MCF-7 cells

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2025-06-17 DOI:10.1016/j.bse.2025.105065

Sheikh Showkat Ahmad, Chandnig Garg, Satwinderjeet Kaur

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引用次数: 0

Abstract

Tragopogon dubius is a biennial herbaceous plant with multiple uses in ethnomedicine. Traditionally consumed as a vegetable, it has been reported to treat various conditions, including wounds, liver dysfunction, kidney disorders, and certain skin diseases. Despite its traditional use, it remains one of the least explored species in terms of biological activity. This study aimed to evaluate the antigenotoxic, antioxidant, and antiproliferative properties of the ethyl acetate extract obtained from the stem and leaves of T. dubius (TrDE), along with eleven fractions (F-1 to F-11) isolated via preparative thin-layer chromatography. Phytochemical profiling was performed using High-Resolution Liquid Chromatography-Mass Spectrometry (HRLC-MS/QTOF/Orbitrap) and Gas Chromatography-Mass Spectrometry (GC-MS). Among the isolated fractions, F-9, F-10, and F-11 showed the highest antioxidant activity at a concentration of 800 μg/mL, as determined by DPPH (IC₅₀ 44.70, 19.90, and 25.35 μg/mL), ABTS (IC₅₀ 64.53, 22.64, and 34.28 μg/mL), and FRAP (IC₅₀ 37.92, 23.85, and 57.96 μg/mL) assays. The F-11 fraction also exhibited the highest total phenolic content (70.00 ± 1.10 mg GAE/g dw), followed by F-10 and F-9, all exceeding the crude extract (33.04 ± 1.10 mg GAE/g dw). Additionally, the TrDE extract displayed genoprotective effects against hydrogen peroxide (H₂O₂)-induced DNA damage in human lymphocytes. In antiproliferative assays, the fractions demonstrated significant cytotoxicity against MCF-7 breast cancer cells in a dose-dependent manner. Notably, fractions F-7, F-10, and F-11 exhibited the highest cytotoxic effects, with GI₅₀ values of 20.14, 35.37, and 33.58 μg/mL, respectively. These findings suggest that T. dubius fractions possess promising therapeutic potential against human breast cancer (MCF-7), warranting further in vivo investigation.

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黄角角蟾对MCF-7细胞的代谢物分析和细胞毒性

龙眼是一种二年生草本植物，具有多种民族医学用途。传统上，它作为一种蔬菜食用，据报道，它可以治疗各种疾病，包括伤口、肝功能障碍、肾脏疾病和某些皮肤病。尽管它的传统用途，但就生物活性而言，它仍然是被探索最少的物种之一。本研究旨在评价从双鱼茎叶中提取的乙酸乙酯提取物（TrDE）及其制备薄层色谱分离的11个组分（F-1 ~ F-11）的抗毒素、抗氧化和抗增殖性能。采用高分辨率液相色谱-质谱法（HRLC-MS/QTOF/Orbitrap）和气相色谱-质谱法（GC-MS）进行植物化学分析。通过DPPH （IC50分别为44.70、19.90和25.35 μg/mL）、ABTS （IC50分别为64.53、22.64和34.28 μg/mL）和FRAP （IC50分别为37.92、23.85和57.96 μg/mL）测定，F-9、F-10和F-11在800 μg/mL浓度下的抗氧化活性最高。总酚含量最高的部位为F-11(70.00±1.10 mg GAE/g dw)，其次为F-10和F-9，均超过粗提物（33.04±1.10 mg GAE/g dw）。此外，TrDE提取物对过氧化氢（H2O2）诱导的人淋巴细胞DNA损伤具有基因保护作用。在抗增殖试验中，该组分对MCF-7乳腺癌细胞显示出明显的细胞毒性，呈剂量依赖性。其中，F-7、F-10和F-11部位的细胞毒作用最强，其GI50值分别为20.14、35.37和33.58 μg/mL。这些发现表明，双歧杆菌部分具有治疗人乳腺癌（MCF-7）的良好潜力，值得进一步的体内研究。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.