Anti-apoptotic effects of the medicinal plant Sterculia setigera in a model of serum deprivation-induced PC12 cells death

IF 2 Q3 NEUROSCIENCES

IBRO Neuroscience Reports Pub Date : 2025-06-16 DOI:10.1016/j.ibneur.2025.06.007

Yendubé T. Kantati , Magloire K. Kodjo , Benjamin Lefranc , Kwashie Eklu-Gadegbeku , Jérôme Leprince , David Vaudry

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Abstract

Sterculia setigera is a medicinal plant of Togolese flora. We have previously reported that S. setigera leaves dry hydroethanolic extract (SSE) protects in vitro cerebellar granule neurons against H₂O₂ and 6-OHDA-induced cell death and in vivo against ethanol neurotoxicity on the cerebellar cortex of 8-day-old Wistar rats. The present study aimed to extend our knowledge of the protective effects of SSE by exploring its anti-apoptotic mechanisms on cultured PC12 cells. Apoptosis was induced by serum depletion before treatment with various concentrations (5–100 µg/mL) of SSE. SSE (20 µg/mL) significantly protected PC12 cells (+51.8 %) against serum depletion-induced PC12 cell death and inhibited Caspase-3/7 (-65.1 %) activity. SSE also significantly increased the expression of the anti-apoptotic factor Bcl-2. Conversely, it repressed the expression of Casp-3, Tp53 and Ddit3. Taken together, these results indicate that inhibition of apoptosis appears to be one of the main mechanisms of S. setigera neuroprotective effects.

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药用植物小檗在血清剥夺诱导的PC12细胞死亡模型中的抗凋亡作用

石菖蒲是多哥的一种药用植物。我们之前报道了五叶草叶干氢乙醇提取物（SSE）在体外保护小脑颗粒神经元免受H2O2和6-羟多巴胺诱导的细胞死亡，并在体内保护8日龄Wistar大鼠小脑皮质免受乙醇神经毒性。本研究旨在通过探索SSE对培养的PC12细胞的抗凋亡机制来扩展我们对其保护作用的认识。不同浓度（5-100 µg/mL）的SSE处理前，用血清去氧诱导细胞凋亡。SSE（20 µg/mL）显著保护PC12细胞（+51.8 %）免受血清消耗诱导的PC12细胞死亡，并抑制Caspase-3/7（-65.1 %）活性。SSE还显著提高了抗凋亡因子Bcl-2的表达。相反，它抑制Casp-3、Tp53和Ddit3的表达。综上所述，这些结果表明，抑制细胞凋亡可能是五味子神经保护作用的主要机制之一。

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