Effect of liquiritin on the expression of BDNF, Bax, and Bcl-2 in the hippocampus of post-stroke depression rats

IF 2.8 Q3 CLINICAL NEUROLOGY

Cerebral circulation - cognition and behavior Pub Date : 2025-01-01 DOI:10.1016/j.cccb.2025.100388

Gui-Liu Yan , Dan Dai , Qiang Zi , Fu-Mei Zhang , Yun Li

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Abstract

Aims

The study intended to explore the therapeutic effect of liquiritin on PSD rats and its role in the pathogenesis of PSD.

Methods

The stroke model was established via middle cerebral artery occlusion, and the PSD model was created using chronic unpredictable mild stress combined with isolated feeding. The expressions of BDNF, Bax, and Bcl-2 proteins in the hippocampus of rats were detected using Western blot and immunofluorescence staining after 6 weeks of modeling.

Results

The weight, sucrose consumption and activity of the PSD rats decreased (P < 0.05) compared with the normal control and stroke groups. On the contrary, the weights of the liquiritin and escitalopram groups increased and their sucrose consumption and activity increased in the open-field test (P < 0.05) compared with the PSD and normal saline (NS) groups.The result of immunofluorescence staining and western-blot showed that BDNF and Bcl-2 increased in the liquiritin group and Bax increased significantly in the stroke and PSD groups (P < 0.05).

Conclusions

Liquiritin is capable of inhibiting neuronal apoptosis in the hippocampus of PSD rats to improve depression symptoms. This improvement may be achieved by reducing the expression of Bax and increasing the expressions of Bcl-2 and BDNF in the hippocampus of PSD rats.

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甘草素对脑卒中后抑郁大鼠海马BDNF、Bax、Bcl-2表达的影响

目的探讨利尿素对PSD大鼠的治疗作用及其在PSD发病机制中的作用。方法采用大脑中动脉闭塞法建立脑卒中模型，采用慢性不可预测轻度应激联合孤立喂养法建立PSD模型。造模6周后，采用Western blot和免疫荧光染色法检测大鼠海马组织中BDNF、Bax、Bcl-2蛋白的表达。结果PSD大鼠体重、糖消耗和活动均明显降低(P <；0.05)，与正常对照组和脑卒中组比较。相反，在大田试验中，利尿素组和艾司西酞普兰组体重增加，蔗糖消耗量和活性增加(P <；0.05)，与生理盐水（NS）组比较。免疫荧光染色和western-blot结果显示，利尿素组BDNF和Bcl-2升高，脑卒中和PSD组Bax升高(P <；0.05)。结论利奎列汀可抑制PSD大鼠海马神经元凋亡，改善抑郁症状。这种改善可能通过降低PSD大鼠海马中Bax的表达，增加Bcl-2和BDNF的表达来实现。

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