Photocatalyzed Spatioselective, Orthogonal, and Covalent Immobilization of Biomolecules onto Cellulose-Based Paper

IF 7.2 2区材料科学 Q2 CHEMISTRY, PHYSICAL

Chemistry of Materials Pub Date : 2025-06-17 DOI:10.1021/acs.chemmater.5c00308

Ingo Bork, Adrian Bloch, Carolin S. Dombrowsky, Dominic Happel, Sejal Kapoor, Susanne S. Klinke, Enis M. Saritas, Lukas Neuenfeld, Tobias Meckel, Markus Biesalski, Harald Kolmar

引用次数: 0

Abstract

In this study, a three-step approach for the immobilization of biomolecules on commercially available filter paper is presented. Photocatalytic coupling of a linker moiety to the paper fibers allows for spatiotemporal control of the immobilization site. An appropriate linker was attached to the biomolecule, allowing catalyst-free, site-specific, and covalent attachment of the biomolecule. Orthogonal linker pairs were evaluated, and five conjugation strategies were identified as providing robust and orthogonal attachment of the desired biomolecule. The suitability of these methods for the generation of complex biofunctionalized papers was demonstrated through premodification of a single sheet of paper with the respective linkers in a spatioselective manner. Application of a mixture of proteins containing the respective linker moieties resulted in successful immobilization of the proteins at the desired sites.

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光催化空间选择、正交和共价固定化生物分子到纤维素基纸上

在这项研究中，一个三步的方法固定化的生物分子在市售滤纸提出。与纸纤维的连接体部分的光催化偶联允许对固定位点进行时空控制。一个合适的连接体被连接到生物分子上，允许生物分子的无催化剂、位点特异性和共价连接。对正交连接对进行了评价，并确定了五种偶联策略，以提供所需生物分子的坚固和正交连接。这些方法对于生成复杂的生物功能化论文的适用性，通过以空间选择性的方式对单张纸进行预先修饰来证明。应用含有相应连接子部分的蛋白质混合物，成功地将蛋白质固定在所需的位点上。

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来源期刊

Chemistry of Materials 工程技术-材料科学：综合

CiteScore

14.10

自引率

5.80%

发文量

929

审稿时长

1.5 months

期刊介绍： The journal Chemistry of Materials focuses on publishing original research at the intersection of materials science and chemistry. The studies published in the journal involve chemistry as a prominent component and explore topics such as the design, synthesis, characterization, processing, understanding, and application of functional or potentially functional materials. The journal covers various areas of interest, including inorganic and organic solid-state chemistry, nanomaterials, biomaterials, thin films and polymers, and composite/hybrid materials. The journal particularly seeks papers that highlight the creation or development of innovative materials with novel optical, electrical, magnetic, catalytic, or mechanical properties. It is essential that manuscripts on these topics have a primary focus on the chemistry of materials and represent a significant advancement compared to prior research. Before external reviews are sought, submitted manuscripts undergo a review process by a minimum of two editors to ensure their appropriateness for the journal and the presence of sufficient evidence of a significant advance that will be of broad interest to the materials chemistry community.