Acupuncture Potentiates anti-PD-1 Efficacy by Promoting CD5+ Dendritic Cells and T Cell-Mediated Tumor Immunity in a Mouse Model of Breast Cancer.

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Xiaoru Xu, Nan Wang, Yufen Li, Shasha Fan, Baohui Mu, Jianxun Zhu
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引用次数: 0

Abstract

Acupuncture has been recognized as a complementary therapy for various malignancies by modulating immune responses. However, the impact of acupuncture on the antitumor immune efficacy of PD-1 inhibitors remains unclear. This study evaluates the effectiveness of acupuncture in conjunction with PD-1 inhibitors in enhancing the antitumor immune response against breast cancer and elucidates the potential molecular mechanisms through RNA-Seq transcriptome analysis in a 4T1 xenograft BALB/c mouse model of breast cancer. The findings indicate that the combination of acupuncture and PD-1 inhibitors significantly impedes tumor development by promoting tumor cell apoptosis and inhibiting tumor growth, angiogenesis, and metastasis. RNA-Seq analysis revealed that differentially expressed genes following acupuncture intervention were primarily enriched in immune response pathways, T cell activation, and cytokine interactions, including immune cell-related CD genes such as CD5, CD4, and CD8. Notably, acupuncture stimulation enhanced CD5 expression, which correlated positively with overall survival in breast cancer patients. Furthermore, the combination treatment led to improved immunity characterized by an increase in CD5+ dendritic cells, as well as CD4+ and CD8+ T cell populations, alongside elevated serum levels of various cytokines (IL-2, IL-6, TNF-α, IFN-γ). Collectively, this study demonstrates that acupuncture intervention enhances the antitumor immune response associated with PD-1 inhibitors, suggesting a promising therapeutic approach for immune checkpoint inhibitor therapy in breast cancer.

针刺通过促进CD5+树突状细胞和T细胞介导的肿瘤免疫在乳腺癌小鼠模型中增强抗pd -1疗效
针灸已被认为是一种补充治疗各种恶性肿瘤通过调节免疫反应。然而,针刺对PD-1抑制剂抗肿瘤免疫效果的影响尚不清楚。本研究评估了针刺联合PD-1抑制剂增强乳腺癌抗肿瘤免疫应答的有效性,并通过RNA-Seq转录组分析阐明了4T1异种移植BALB/c乳腺癌小鼠模型中潜在的分子机制。研究结果表明,针刺联合PD-1抑制剂通过促进肿瘤细胞凋亡,抑制肿瘤生长、血管生成和转移,显著阻碍肿瘤的发展。RNA-Seq分析显示,针刺干预后的差异表达基因主要富集于免疫应答途径、T细胞活化和细胞因子相互作用,包括免疫细胞相关的CD基因,如CD5、CD4和CD8。值得注意的是,针刺刺激可增强CD5的表达,并与乳腺癌患者的总生存率呈正相关。此外,联合治疗可改善免疫,其特征是CD5+树突状细胞、CD4+和CD8+ T细胞群增加,同时血清中各种细胞因子(IL-2、IL-6、TNF-α、IFN-γ)水平升高。总的来说,本研究表明针灸干预增强了与PD-1抑制剂相关的抗肿瘤免疫反应,为免疫检查点抑制剂治疗乳腺癌提供了一种有希望的治疗方法。
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来源期刊
CiteScore
5.80
自引率
3.40%
发文量
172
审稿时长
3 months
期刊介绍: This timely publication reports and reviews current findings on the effects of nutrition on the etiology, therapy, and prevention of cancer. Etiological issues include clinical and experimental research in nutrition, carcinogenesis, epidemiology, biochemistry, and molecular biology. Coverage of therapy focuses on research in clinical nutrition and oncology, dietetics, and bioengineering. Prevention approaches include public health recommendations, preventative medicine, behavior modification, education, functional foods, and agricultural and food production policies.
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