Evaluation of Platinum Resistance or Sensitivity Markers by Analysis of Cell Block Specimens of Ascites Fluid in High-Grade Serous Carcinoma.

IF 1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Journal of Cytology Pub Date : 2025-04-01 Epub Date: 2025-05-29 DOI:10.4103/joc.joc_152_24

Rinako Ogawa, Rintaro Ohe, Takumi Kitaoka, Tsuyoshi Ohta, Satoru Nagase, Mitsuru Futakuchi

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Abstract

Background: Platinum resistance in cancer cells plays an important role in the recurrence and progression of high-grade serous carcinoma (HGSC). Advanced or recurrent HGSC often presents with substantial ascites fluid retention.

Aims: To demonstrate that ascites fluid sampling could help evaluate the predictive markers for platinum resistance or sensitivity in peritoneal lesions of HGSC, we compared the expression of platinum response markers of cancer cells in ascites fluid with that in peritoneal lesions.

Materials and methods: Thirty samples from 10 HGSC patients were collected, including formalin-fixed paraffin-embedded specimens obtained from peritoneal lesions and primary ovarian lesions, and cell block specimens obtained from ascites fluid. The morphology of the cancer cells was evaluated by hematoxylin and eosin staining. The expression of predictive markers for platinum response in cancer cells was evaluated by immunohistochemistry for excision repair cross-complementation group 1 (ERCC1) and Schlafen 11 (SLFN11).

Results: The morphology of cancer cells in cell blocks (CBs) was similar to that in peritoneal and primary lesions. The expression of ERCC1 and SLFN11 in cancer cells in CBs was positively correlated with that in peritoneal lesions. These results indicated that CBs of ascites fluid would be useful for evaluating the expression of predictive markers for platinum resistance and sensitivity.

Conclusion: This study demonstrated that HGSC cells in ascites fluid may reflect the expression of predictive markers for platinum resistance or sensitivity in cancer cells in peritoneal metastatic lesions.

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高级别浆液性癌腹水细胞块分析评价铂耐药或敏感性标志物。

背景：癌细胞的铂耐药在高级别浆液性癌（HGSC）的复发和进展中起重要作用。晚期或复发性造血干细胞常表现为大量腹水潴留。目的：为了证明腹水取样可以帮助评估HGSC腹膜病变中铂耐药性或敏感性的预测标志物，我们比较了腹水中癌细胞铂反应标志物与腹膜病变中铂反应标志物的表达。材料和方法：收集10例HGSC患者的30份标本，包括腹膜病变和卵巢原发病变的福尔马林固定石蜡包埋标本和腹水细胞块标本。苏木精染色、伊红染色观察癌细胞形态。通过免疫组织化学方法评估肿瘤细胞中铂反应预测标志物在切除修复交叉互补组1 （ERCC1）和Schlafen 11 （SLFN11）中的表达。结果：细胞块（CBs）中癌细胞形态与腹膜及原发病变相似。肿瘤细胞中ERCC1和SLFN11的表达与腹膜病变细胞中ERCC1和SLFN11的表达呈正相关。这些结果表明，腹水CBs可用于评估铂耐药性和敏感性的预测标志物的表达。结论：本研究表明腹水中的HGSC细胞可能反映了腹膜转移病变中癌细胞对铂的耐药性或敏感性的预测标志物的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Cytology MEDICAL LABORATORY TECHNOLOGY-

CiteScore

1.80

自引率

7.70%

发文量

审稿时长

46 weeks

期刊介绍： The Journal of Cytology is the official Quarterly publication of the Indian Academy of Cytologists. It is in the 25th year of publication in the year 2008. The journal covers all aspects of diagnostic cytology, including fine needle aspiration cytology, gynecological and non-gynecological cytology. Articles on ancillary techniques, like cytochemistry, immunocytochemistry, electron microscopy, molecular cytopathology, as applied to cytological material are also welcome. The journal gives preference to clinically oriented studies over experimental and animal studies. The Journal would publish peer-reviewed original research papers, case reports, systematic reviews, meta-analysis, and debates.