Highly specific virus-host linkages in mesothermophilic and circumneutral hot springs microbial communities.

Abstract

Microbial communities in hot springs are distributed globally and have been extensively characterized regarding their diversity and composition. However, most studies have focused on cellular microbes, with relatively few addressing viruses, and even fewer examining virus-host ecology. Furthermore, research on viral communities and virus-host interactions has predominantly targeted extremely thermophilic environments, leaving mesothermophilic (40-80 °C) and circumneutral (pH 6-8) hot spring less explored. Here, we describe virus-host linkages using 37 metagenomic datasets obtained from 14 mesothermophilic and circumneutral hot springs. A parallel analysis of hot spring habitats-sediment, microbial mat, and water-employing diversity metrics, network analyses, and machine learning for virus-host pair identification revealed that viral communities appear to be more endemic than their hosts. Additionally, certain host taxa were identified as differentially abundant across habitats, some corresponding to specific hubs in the network analyses. Most viruses were linked to hosts within the same metagenome, with only a small fraction classified as cosmopolitan. Furthermore, nearly all virus-host pairs were exclusive to a single habitat type-sediment, microbial mat, or water. These findings suggests that virus-host linkages in mesothermophilic and circumneutral hot springs are highly specific, reflecting ecological adaptation within habitat niches.