Collagen fibers as an indicator of postmortem interval in non-gravid uterus, prostate, and skeletal muscles of adult albino rats.

IF 1.5 4区医学 Q1 LAW

Medicine, Science and the Law Pub Date : 2025-06-16 DOI:10.1177/00258024251348721

Nermin Nabil Fayed, Nadia A Kotb, Samaa Samir Kamar, Doaa Tawfik

{"title":"Collagen fibers as an indicator of postmortem interval in non-gravid uterus, prostate, and skeletal muscles of adult albino rats.","authors":"Nermin Nabil Fayed, Nadia A Kotb, Samaa Samir Kamar, Doaa Tawfik","doi":"10.1177/00258024251348721","DOIUrl":null,"url":null,"abstract":"<p><p>Determination of postmortem interval (PMI) is a fundamental aspect of forensic medicine, aiding in the reconstruction of the time of death in medico-legal investigations. The present study aims to compare gender-based postmortem changes and investigates the role of collagen fibers as a potential indicator of PMI in albino rats. Eighteen male and nine female adult albino rats were examined. Three prostates, three uterine horns, and the quadriceps muscle of the hind limb of each animal were collected at six time points (0, 6, 24, 36, 48, and 144 h postmortem) after scarifying rats by neck dislocation. Morphological analysis revealed progressive softening of the organs with darker discoloration with increasing PMI. Histological examination using hematoxylin and eosin staining showed cellular degradation up to 48 h PM. Masson's trichrome staining highlighted the persistence of collagen fibers up to 144 h. Notably, male prostates exhibited greater resistance to postmortem degradation compared to female uteri, suggesting that potential sex identification by gonads is possible up to 48 h PM.</p>","PeriodicalId":18484,"journal":{"name":"Medicine, Science and the Law","volume":" ","pages":"258024251348721"},"PeriodicalIF":1.5000,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medicine, Science and the Law","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/00258024251348721","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"LAW","Score":null,"Total":0}

引用次数: 0

Abstract

Determination of postmortem interval (PMI) is a fundamental aspect of forensic medicine, aiding in the reconstruction of the time of death in medico-legal investigations. The present study aims to compare gender-based postmortem changes and investigates the role of collagen fibers as a potential indicator of PMI in albino rats. Eighteen male and nine female adult albino rats were examined. Three prostates, three uterine horns, and the quadriceps muscle of the hind limb of each animal were collected at six time points (0, 6, 24, 36, 48, and 144 h postmortem) after scarifying rats by neck dislocation. Morphological analysis revealed progressive softening of the organs with darker discoloration with increasing PMI. Histological examination using hematoxylin and eosin staining showed cellular degradation up to 48 h PM. Masson's trichrome staining highlighted the persistence of collagen fibers up to 144 h. Notably, male prostates exhibited greater resistance to postmortem degradation compared to female uteri, suggesting that potential sex identification by gonads is possible up to 48 h PM.

查看原文本刊更多论文

胶原纤维在成年白化大鼠非妊娠子宫、前列腺和骨骼肌中作为死后时间的指标。

死亡间隔的确定是法医学的一个基本方面，有助于在法医调查中重建死亡时间。本研究旨在比较基于性别的死后变化，并探讨胶原纤维作为白化大鼠PMI的潜在指标的作用。研究了18只雄性和9只雌性成年白化大鼠。于颈脱位瘢痕大鼠死后6个时间点（0、6、24、36、48、144 h）采集3只前列腺、3只子宫角和后肢股四头肌。形态学分析显示，随着PMI的增加，器官逐渐软化，颜色变深。苏木精和伊红染色组织学检查显示细胞降解至48小时PM。马松三色染色显示胶原纤维的持久性长达144小时。值得注意的是，与女性子宫相比，男性前列腺对死后降解表现出更强的抵抗力，这表明性腺可能在48小时前进行潜在的性别识别。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Medicine, Science and the Law 医学-医学：法

CiteScore

2.90

自引率

6.70%

发文量

审稿时长

>12 weeks

期刊介绍： Medicine, Science and the Law is the official journal of the British Academy for Forensic Sciences (BAFS). It is a peer reviewed journal dedicated to advancing the knowledge of forensic science and medicine. The journal aims to inform its readers from a broad perspective and demonstrate the interrelated nature and scope of the forensic disciplines. Through a variety of authoritative research articles submitted from across the globe, it covers a range of topical medico-legal issues. The journal keeps its readers informed of developments and trends through reporting, discussing and debating current issues of importance in forensic practice.