Osteoinductivity performance dicalcium phosphate dihydrate-coated porous β-tricalcium phosphate granules in adipose-derived mesenchymal stem cells.

IF 1.9 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Dental materials journal Pub Date : 2025-06-14 DOI:10.4012/dmj.2024-310

Khairul Anuar Shariff, Alexander Patera Nugraha, Ahmed Hafedh Mohammed Mohammed, Ida Bagus Narmada, I Gusti Aju Wahju Ardani, Nastiti Faradilla Ramadhani, Ratri Maya Sitalaksmi, Wibi Riawan, Helen Susilowati, Suryo Kuncorojakti, Bramasta Nugraha, Guang Hong

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Abstract

Elucidate the dicalcium phosphate dihydrate (DCPD)-coated-beta-tricalcium phosphate (β-TCP) porous granules osteoinductivity performance in adipose-derived mesenchymal stem cells (ADMSCs). β-TCP porous was made by adding the sodium chloride (NaCl) powders with the dry powder of DCPD and CaCO₃ in the concentrations 40:60 (by wt%). Then, DCPD layer will be formed on porous β-TCP granules by immersing the granules in an acidic calcium phosphate solution. ADMSCs cultured was used to examine the biocompability of 300-600 µm and 600-1,000 µm DCPD-coated-porous β-TCP by means of microculture tetrazolium technique (MTT assay) and scratch assay. Bone morphogenic protein-2 (BMP-2), Osterix, Runt-related transcription factor-2 (Runx2), Alkaline phosphatase (ALP), Osteopontin, Osteonectin expression were investigated. The DCPD-coated porous β-TCP granules were culture together with ADMSCs is biocompatible. BMP-2, Runx2, Osterix, ALP, Osteopontin, Osteonectin expression in ADMSCs after culture together with DCPD-coated-β-TCP significantly enhanced. DCPD-coated-porous-β-TCP granules have good biocompatibility and high osteoconductivity in ADMSCs.

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二水磷酸二钙包被多孔β-磷酸三钙颗粒在脂肪源间充质干细胞中的骨诱导性能。

阐明二水磷酸二钙（DCPD）包被-磷酸三钙（β-TCP）多孔颗粒在脂肪源性间充质干细胞（ADMSCs）中的成骨性能。将DCPD和CaCO3的干粉以40:60 （wt%）的比例加入氯化钠（NaCl）粉中制备β-TCP多孔材料。然后将多孔β-TCP颗粒浸泡在酸性磷酸钙溶液中，形成DCPD层。采用微培养四氮唑法（MTT法）和划痕法检测培养的ADMSCs 300-600µm和600- 1000µm dcpd涂层多孔β-TCP的生物相容性。检测骨形态发生蛋白-2 （BMP-2）、Osterix、runt相关转录因子-2 （Runx2）、碱性磷酸酶（ALP）、骨桥蛋白（Osteopontin）、骨连接蛋白（Osteonectin）的表达。dcpd包被的多孔β-TCP颗粒与ADMSCs一起培养，具有生物相容性。BMP-2、Runx2、Osterix、ALP、Osteopontin、Osteonectin在ADMSCs中与dcpd包被的-β-TCP一起培养后表达显著增强。dcpd包覆多孔β- tcp颗粒在ADMSCs中具有良好的生物相容性和高的骨导电性。

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来源期刊

Dental materials journal 医学-材料科学：生物材料

CiteScore

4.60

自引率

4.00%

发文量

102

审稿时长

3 months

期刊介绍： Dental Materials Journal is a peer review journal published by the Japanese Society for Dental Materials and Devises aiming to introduce the progress of the basic and applied sciences in dental materials and biomaterials. The dental materials-related clinical science and instrumental technologies are also within the scope of this journal. The materials dealt include synthetic polymers, ceramics, metals and tissue-derived biomaterials. Forefront dental materials and biomaterials used in developing filed, such as tissue engineering, bioengineering and artificial intelligence, are positively considered for the review as well. Recent acceptance rate of the submitted manuscript in the journal is around 30%.