Whole genome sequence analysis of a novel Streptomyces sp. BG6AG reveals its lipase production potential: optimization of the process through Taguchi experimental design.

IF 2.6 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-07-01 Epub Date: 2025-06-11 DOI:10.1007/s13205-025-04369-4

Zahra Parwez, Subhransu Sekhar Behera, Ananta Narayan Panda, Sudhansu Kumar Gouda, Lopamudra Ray

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Abstract

This study reports a lipase-producing Streptomyces strain BG6AG isolated from Chilika Lake, Odisha, India. Whole genome analysis revealed a genome size of 7,565,774 base pairs with a GC content of 72.2%. The genomic analysis identified 6740 coding sequences, 59 tRNA, and 3 rRNA molecules, utilizing bioinformatics tools such as KEGG, COG, antiSMASH, TYGS, CGView, RGI, and RAST. Biochemical tests confirmed the presence of lipase enzymes in the organism. The lipase production was optimized using Taguchi statistical design, a robust method that enhances experimental efficiency. The results indicated that the optimal medium for lipase production is Minimal Salt Medium (MSM) supplemented with tributyrin, arabinose, asparagine, and Mn²⁺, adjusted to pH 9. Optimization of media has resulted in two-fold increase in lipase production. The findings contribute to the understanding of Streptomyces sp. BG6AG's genomic features and its potential as a source of industrially relevant enzymes.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04369-4.

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新型链霉菌BG6AG的全基因组序列分析揭示了其脂肪酶生产潜力：通过田口实验设计优化工艺。

本研究报道了一株产脂肪酶链霉菌BG6AG，分离自印度奥里萨邦奇里卡湖。全基因组分析显示其基因组大小为7,565,774个碱基对，GC含量为72.2%。基因组分析利用KEGG、COG、antiSMASH、TYGS、CGView、RGI和RAST等生物信息学工具，鉴定出6740个编码序列、59个tRNA和3个rRNA分子。生化试验证实了生物体中存在脂肪酶。采用田口统计设计优化脂肪酶产量，提高了实验效率。结果表明，生产脂肪酶的最佳培养基为添加三丁酸甘油酯、阿拉伯糖、天冬酰胺和Mn2+的最低盐培养基（MSM），调节pH为9。培养基的优化使脂肪酶的产量增加了两倍。这些发现有助于了解链霉菌BG6AG的基因组特征及其作为工业相关酶来源的潜力。补充资料：在线版本包含补充资料，下载地址：10.1007/s13205-025-04369-4。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.