Microbial synergy between Rhodospirillum rubrum and Acetobacterium woodii enables anaerobic CO conversion to polyhydroxyalkanoates.

IF 9.3 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Green Chemistry Pub Date : 2025-05-28 DOI:10.1039/d5gc01092f

Timon M Torres Ruano, Martijn Diender, Diana Z Sousa

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引用次数: 0

Abstract

The high cost of traditional substrates has hindered the large-scale adoption of polyhydroxyalkanoates (PHAs) as sustainable alternatives to petrochemical plastics. One-carbon (C1) substrates like carbon monoxide (CO) offer a low-cost, sustainable feedstock, but efficient biocatalytic systems for their conversion to PHAs have been lacking. Here, we report the first successful anaerobic production of PHAs from CO using a synthetic co-culture of Rhodospirillum rubrum and Acetobacterium woodii. In this system, R. rubrum catalyzes the water-gas shift reaction, converting CO into H₂ and CO₂. A. woodii subsequently transforms these products into acetate, serving as an organic carbon source for PHA accumulation by R. rubrum. Neither organism, in monoculture, was able to grow on CO alone, underscoring the importance of the microbial synergy. While continuous cultivation in chemostats proved unstable, fed-batch cultivation achieved a PHA production rate of 58 ± 11 mg L_medium ^-1 day^-1 with a final PHA content of 38 ± 5% (dry weight). This study introduces a pioneering anaerobic route for PHA synthesis from CO, representing a significant advance toward sustainable PHA production from C1 substrates.

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红红螺旋菌和伍地乙杆菌之间的微生物协同作用使厌氧CO转化为聚羟基烷酸酯。

传统基材的高成本阻碍了聚羟基烷酸酯（PHAs）作为石化塑料可持续替代品的大规模采用。一碳（C1）底物，如一氧化碳（CO）提供了一种低成本、可持续的原料，但缺乏有效的生物催化系统将其转化为pha。在这里，我们报告了第一次成功的从CO厌氧生产相芳烃使用合成共培养红红螺旋菌和伍迪醋酸杆菌。在该体系中，红草催化水气转换反应，将CO转化为H2和CO2。伍地木随后将这些产物转化为乙酸，作为红草积累PHA的有机碳源。在单一栽培中，这两种生物都不能单独依靠CO生长，这强调了微生物协同作用的重要性。虽然在恒温器中连续培养不稳定，但分批补料培养的PHA产量为58±11 mg Lmedium -1 day-1，最终PHA含量为38±5%（干重）。本研究介绍了一种由CO合成PHA的开创性厌氧途径，代表了从C1底物可持续生产PHA的重大进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Green Chemistry 化学-化学综合

CiteScore

16.10

自引率

7.10%

发文量

677

审稿时长

1.4 months

期刊介绍： Green Chemistry is a journal that provides a unique forum for the publication of innovative research on the development of alternative green and sustainable technologies. The scope of Green Chemistry is based on the definition proposed by Anastas and Warner (Green Chemistry: Theory and Practice, P T Anastas and J C Warner, Oxford University Press, Oxford, 1998), which defines green chemistry as the utilisation of a set of principles that reduces or eliminates the use or generation of hazardous substances in the design, manufacture and application of chemical products. Green Chemistry aims to reduce the environmental impact of the chemical enterprise by developing a technology base that is inherently non-toxic to living things and the environment. The journal welcomes submissions on all aspects of research relating to this endeavor and publishes original and significant cutting-edge research that is likely to be of wide general appeal. For a work to be published, it must present a significant advance in green chemistry, including a comparison with existing methods and a demonstration of advantages over those methods.