Kadcoccinoids A-K, triterpenoids from Kadsura coccinea with PCSK9 expression inhibitory activities

IF 3.4 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Phytochemistry Pub Date : 2025-06-14 DOI:10.1016/j.phytochem.2025.114584

Yelin Duan , Tong Chen , Pengju Guo , Chenyue Li , Yihan Chen , Mingge Zhang , Mengkun Yan , Wenqiong Wang , Lijiang Xuan

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Abstract

Two undescribed protostane-type triterpenoids (1–2), and nine undescribed lanostane-type triterpenoids (3–11), along with nine known compounds (12–20) were isolated from the stems of Kadsura coccinea. Their chemical structures were elucidated by extensive spectroscopic analyses, Mosher's method, X-ray crystallography, and electronic circular dichroism calculations. Kadcoccinoid A (1) possesses an undescribed 6/6/8-fused carbocyclic core containing a rare bicyclo [4.2.1] nonan-9-one system and proposed plausible biosynthetic pathway. Additionally, kadcoccinoid B (2) significantly decreased PCSK9 protein levels in the medium of HepG2 cells at 10 μM with an inhibition rate of 78.4 % and increased LDL uptake at 5 and 10 μM, while compounds 12–14 showed moderate PCSK9 inhibitory activities.

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具有PCSK9表达抑制活性的三萜类Kadsura coccinoids A-K

从鸭尾草茎中分离得到2个未描述的原烷型三萜（1-2）和9个未描述的羊毛烷型三萜（3-11），以及9个已知化合物（12-20）。通过广泛的光谱分析、Mosher方法、x射线晶体学和电子圆二色性计算，阐明了它们的化学结构。Kadcoccinoid A(1)具有未描述的6/6/8融合的碳环核心，包含罕见的双环[4.2.1]nonan-9- 1系统，并提出了合理的生物合成途径。此外，kadcoccinoid B(2)在HepG2细胞10 μM的培养基中显著降低PCSK9蛋白水平，抑制率为78.4%，并增加5和10 μM的LDL摄取，而化合物12-14表现出中等的PCSK9抑制活性。

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.