Simplified synthetic community reveals the enrichment of phthalate-degrading bacteria in maize rhizosphere enhancing phthalate degradation

IF 7.4 2区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of Environmental Chemical Engineering Pub Date : 2025-06-07 DOI:10.1016/j.jece.2025.117495

Jia-Lu Wei , Yu-Hong Huang , Jun-Hua Li , Huixiong Lü , Hai-Ming Zhao , Lei Xiang , Hui Li , Yan-Wen Li , Ce-Hui Mo , Quan-Ying Cai , Qing X. Li

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引用次数: 0

Abstract

Agricultural soil contamination by toxic pollutants like phthalates (PAEs) threatens soil health and food safety, and urgently requires efficient remediation. Rhizoremediation has emerged as a promising approach, leveraging the biodegradation capabilities of microbes in rhizosphere to enhance PAE removal. However, how these PAE-degraders function and cooperate in rhizosphere contributing to PAE degradation remain poorly understood. This study employed the strategy of synthetic community to explore how maize rhizosphere enrich PAE-degrading bacteria and regulate microbial cooperation for enhancing PAE degradation. Four PAE-degrading bacterial strains (i.e., Paenarthrobacter sp. R6, Mycobacterium sp. R14, Rhizobium sp. R15, and Arthrobacter sp. R25) which were isolated from maize rhizosphere showed varied degradation efficiencies (14 % - 97 %) for recalcitrant di-(2-ethylhexyl) phthalate (DEHP). These strains were constructed into a simplified synthetic community SynCom-4 which exhibited superior DEHP degradation capacity compared to individual strains, and could completely degrade DEHP through extra-/intra-cellular enzyme reaction and metabolic exchange. Recolonization experiments indicated that SynCom-4 more significantly enhanced DEHP dissipation in rhizosphere than those in bulk soil (68.7 % vs. 65.2 % in bulk soil; 78 % vs. 71.7 % in rhizosphere by 50 d). SynCom-4 showed higher colonization efficiency in rhizosphere than bulk soil. Enrichment of SynCom-4 and their cooperative partners (e.g., genera Chitinophaga and Sphingobium) in rhizosphere facilitated DEHP degradation with 17 % - 39 % higher DEHP dissipation percentages than those in bulk soil without inoculation. In conclusion, enrichment of PAE-degrading bacteria and the microbial cooperations in maize rhizosphere remarkably contributed to enhance PAE degradation, which were significant for rhizoremediation of large-scale PAE-polluted agricultural soil.

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简化的合成群落揭示了玉米根际中邻苯二甲酸酯降解菌的富集促进了邻苯二甲酸酯的降解

邻苯二甲酸盐（PAEs）等有毒污染物对农业土壤的污染威胁着土壤健康和食品安全，迫切需要有效的修复措施。根际修复已经成为一种很有前途的方法，利用根际微生物的生物降解能力来增强PAE的去除。然而，这些PAE降解物如何在根际发挥作用并相互合作，从而促进PAE的降解仍然知之甚少。本研究采用合成群落策略，探讨玉米根际如何富集降解PAE的细菌，调控微生物间的合作以促进PAE的降解。从玉米根际分离的4株降解pae的细菌（即Paenarthrobacter sp. R6、Mycobacterium sp. R14、Rhizobium sp. R15和Arthrobacter sp. R25）对难降解邻苯二甲酸二（2-乙基己基）酯（DEHP）的降解效率不同（14 % ~ 97 %）。将这些菌株构建成一个简化的合成群落SynCom-4，该群落具有比单个菌株更强的DEHP降解能力，可以通过胞外/胞内酶反应和代谢交换完全降解DEHP。再定殖试验表明，SynCom-4对根际DEHP耗散的促进作用显著高于散装土（68.7% % vs. 65.2% %）；78 % vs. 71.7 %，相差50 d)。SynCom-4在根际的定殖效率高于散装土。在根际富集SynCom-4及其合作伙伴（Chitinophaga属和Sphingobium属）促进了DEHP的降解，其DEHP耗散率比未接种的散土高17 % ~ 39 %。综上所述，玉米根际中PAE降解菌的富集和微生物的协同作用显著促进了PAE的降解，这对大规模修复PAE污染的农业土壤具有重要意义。

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来源期刊

Journal of Environmental Chemical Engineering Environmental Science-Pollution

CiteScore

11.40

自引率

6.50%

发文量

2017

审稿时长

27 days

期刊介绍： The Journal of Environmental Chemical Engineering (JECE) serves as a platform for the dissemination of original and innovative research focusing on the advancement of environmentally-friendly, sustainable technologies. JECE emphasizes the transition towards a carbon-neutral circular economy and a self-sufficient bio-based economy. Topics covered include soil, water, wastewater, and air decontamination; pollution monitoring, prevention, and control; advanced analytics, sensors, impact and risk assessment methodologies in environmental chemical engineering; resource recovery (water, nutrients, materials, energy); industrial ecology; valorization of waste streams; waste management (including e-waste); climate-water-energy-food nexus; novel materials for environmental, chemical, and energy applications; sustainability and environmental safety; water digitalization, water data science, and machine learning; process integration and intensification; recent developments in green chemistry for synthesis, catalysis, and energy; and original research on contaminants of emerging concern, persistent chemicals, and priority substances, including microplastics, nanoplastics, nanomaterials, micropollutants, antimicrobial resistance genes, and emerging pathogens (viruses, bacteria, parasites) of environmental significance.