Olink proteomics reveals TNFRSF9 as a biomarker for abdominal aortic aneurysms

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-06-05 DOI:10.1016/j.isci.2025.112828

Runze Chang , Heng Wang , Chuanlong Lu , Jinshan Chen , Yaling Li , Maolin Qiao , Siqi Gao , Lizheng Li , Keyi Fan , Ruijing Zhang , Honglin Dong

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Abstract

Abdominal aortic aneurysm (AAA) is a serious cardiovascular disease associated with chronic inflammation. The purpose of this study was to use the Olink proteomics to reveal serum inflammatory markers in AAA. We examined the expression levels of 92 inflammation-related proteins in patients with AAA (n = 18) and healthy individuals (n = 10) using the Olink proximity extension assay (PEA) inflammatory plate. Olink proteomics identified 38 differential proteins. Combined analysis of Olink proteomics and GSE183464 showed interleukin-6 (IL-6) and tumor necrosis factor receptor superfamily member 9 (TNFRSF9) were upregulated at both gene and protein levels in AAA patients. The ELISA results were consistent with the Olink proteomics results, and the receiver operating characteristic (ROC) curve analysis revealed that the binding of TNFRSF9 and IL-6 has high diagnostic value (Olink AUC = 0.9056; ELISA AUC = 0.950). Subsequently, elevated TNFRSF9 expression in AAA was confirmed by animal models, suggesting that TNFRSF9 may serve as a potential biomarker for AAA.

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链接蛋白质组学显示TNFRSF9是腹主动脉瘤的生物标志物

腹主动脉瘤（AAA）是一种与慢性炎症相关的严重心血管疾病。本研究的目的是使用Olink蛋白质组学来揭示AAA患者的血清炎症标志物。我们使用Olink接近扩展试验（PEA）炎症板检测了AAA患者（n = 18）和健康个体（n = 10）中92种炎症相关蛋白的表达水平。Olink蛋白质组学鉴定出38种差异蛋白。Olink蛋白组学和GSE183464联合分析显示，在AAA患者中，白细胞介素-6 （IL-6）和肿瘤坏死因子受体超家族成员9 （TNFRSF9）的基因和蛋白水平均上调。ELISA结果与Olink蛋白组学结果一致，受试者工作特征（ROC）曲线分析显示TNFRSF9与IL-6结合具有较高的诊断价值(Olink AUC = 0.9056；Elisa auc = 0.950)。随后，动物模型证实了TNFRSF9在AAA中的表达升高，提示TNFRSF9可能是AAA的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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