Terpene-Based Eutectic Solvent Microdroplets: A Strategy to Combat Antibiotic-Resistant Helicobacter pylori

IF 3.9 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Langmuir Pub Date : 2025-06-03 DOI:10.1021/acs.langmuir.5c0109410.1021/acs.langmuir.5c01094

Marek Brzeziński*, Magdalena Chmiela, Matias Picchio, Marcelo Calderón and Weronika Gonciarz*,

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引用次数: 0

Abstract

Terpene-based therapeutic eutectic solvents (THEES) represent an innovative class of green solvents with intrinsic antimicrobial properties, offering new potential for sustainable solutions in microbial infection treatment. This study introduces the first application of THEES-based microdroplets targeting Helicobacter pylori (H. pylori), a critical global health concern due to rising antibiotic resistance. Using microfluidic technology, we developed oil-in-water (O/W) emulsion droplets from three distinct THEES systems: menthol/thymol, menthol/lidocaine, and menthol/eucalyptol. These droplets were generated via a flow-focusing glass-capillary microfluidic device, with a 10 wt % poly(vinyl alcohol) (PVA) aqueous phase and THEES as the organic phase. The presence of surfactant in the system is expected to diminish the integration of bacterial membranes and improve the antimicrobial efficiency of the emulsion droplets compared to THEES alone. Biocompatibility was first evaluated via metabolic activity on L929 mouse fibroblasts, followed by in vitro testing evaluation against both standard and antibiotic-resistant H. pylori strains. This novel platform THEES-based microdroplets offers a sustainable and potentially transformative strategy for addressing H. pylori infections, providing a breakthrough in combating antibiotic resistance and can potentially prevent the development of resistance if it is used as an alternative to antibiotics.

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萜烯基共熔溶剂微滴：对抗耐抗生素幽门螺杆菌的策略

萜烯基治疗共晶溶剂（THEES）代表了一类具有固有抗菌特性的创新绿色溶剂，为微生物感染治疗的可持续解决方案提供了新的潜力。本研究首次介绍了基于thees的微滴靶向幽门螺杆菌（H. pylori）的应用，幽门螺杆菌是由于抗生素耐药性上升而引起的一个重要的全球健康问题。利用微流体技术，我们从三种不同的THEES体系中开发出水包油（O/W）乳液滴：薄荷醇/百里香酚，薄荷醇/利多卡因和薄荷醇/桉树精油。这些液滴是通过流动聚焦玻璃毛细管微流控装置产生的，其中10%的聚乙烯醇（PVA）水相和有机相为THEES。与单独使用THEES相比，表面活性剂在体系中的存在有望减少细菌膜的整合并提高乳状液滴的抗菌效率。首先通过L929小鼠成纤维细胞的代谢活性来评估生物相容性，然后进行标准和耐药幽门螺杆菌菌株的体外测试评估。这种基于微滴的新型平台为解决幽门螺杆菌感染提供了一种可持续的、潜在的变革性策略，为对抗抗生素耐药性提供了突破，如果将其用作抗生素的替代品，可能会阻止耐药性的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Langmuir 化学-材料科学：综合

CiteScore

6.50

自引率

10.30%

发文量

1464

审稿时长

2.1 months

期刊介绍： Langmuir is an interdisciplinary journal publishing articles in the following subject categories: Colloids: surfactants and self-assembly, dispersions, emulsions, foams Interfaces: adsorption, reactions, films, forces Biological Interfaces: biocolloids, biomolecular and biomimetic materials Materials: nano- and mesostructured materials, polymers, gels, liquid crystals Electrochemistry: interfacial charge transfer, charge transport, electrocatalysis, electrokinetic phenomena, bioelectrochemistry Devices and Applications: sensors, fluidics, patterning, catalysis, photonic crystals However, when high-impact, original work is submitted that does not fit within the above categories, decisions to accept or decline such papers will be based on one criteria: What Would Irving Do? Langmuir ranks #2 in citations out of 136 journals in the category of Physical Chemistry with 113,157 total citations. The journal received an Impact Factor of 4.384*. This journal is also indexed in the categories of Materials Science (ranked #1) and Multidisciplinary Chemistry (ranked #5).