{"title":"Chromosome-level genome assembly of the aphid Megoura crassicauda.","authors":"Tianyu Huang, Xu Cheng, Bing Wang, Guirong Wang","doi":"10.1038/s41597-025-05328-7","DOIUrl":null,"url":null,"abstract":"<p><p>Megoura crassicauda (Hemiptera: Aphididae) is a major pest species that inflicts significant damage on various legume crops worldwide, causing substantial global economic losses. In this study, we present a chromosome-scale genome assembly of M. crassicauda. By integrating PacBio long-read sequencing, Illumina short-read sequencing, and Hi-C scaffolding techniques, we constructed a genome assembly spanning 424.45 Mb genome. Approximately 93.74% of the assembly was successfully anchored into five scaffolds, with contig and scaffold N50 reaching 5.55 Mb and 103.55 Mb, respectively. The genome completeness, as evaluated by BUSCO, achieved a completeness score of 97.75%. Additionally, a total of 14,717 protein-coding genes were identified. This high-quality genome assembly of M. crassicauda serves as a valuable genomic resource, facilitating further studies into the ecological adaptations of M. crassicauda and the development of pest control strategies.</p>","PeriodicalId":21597,"journal":{"name":"Scientific Data","volume":"12 1","pages":"994"},"PeriodicalIF":6.9000,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12166070/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientific Data","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1038/s41597-025-05328-7","RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Megoura crassicauda (Hemiptera: Aphididae) is a major pest species that inflicts significant damage on various legume crops worldwide, causing substantial global economic losses. In this study, we present a chromosome-scale genome assembly of M. crassicauda. By integrating PacBio long-read sequencing, Illumina short-read sequencing, and Hi-C scaffolding techniques, we constructed a genome assembly spanning 424.45 Mb genome. Approximately 93.74% of the assembly was successfully anchored into five scaffolds, with contig and scaffold N50 reaching 5.55 Mb and 103.55 Mb, respectively. The genome completeness, as evaluated by BUSCO, achieved a completeness score of 97.75%. Additionally, a total of 14,717 protein-coding genes were identified. This high-quality genome assembly of M. crassicauda serves as a valuable genomic resource, facilitating further studies into the ecological adaptations of M. crassicauda and the development of pest control strategies.
期刊介绍：
Scientific Data is an open-access journal focused on data, publishing descriptions of research datasets and articles on data sharing across natural sciences, medicine, engineering, and social sciences. Its goal is to enhance the sharing and reuse of scientific data, encourage broader data sharing, and acknowledge those who share their data.
The journal primarily publishes Data Descriptors, which offer detailed descriptions of research datasets, including data collection methods and technical analyses validating data quality. These descriptors aim to facilitate data reuse rather than testing hypotheses or presenting new interpretations, methods, or in-depth analyses.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
